Structure and chain conformation of a (1 → 6)-α-D-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative

被引：101
作者：
Cui, Hengxiang [1 ,2 ]
Liu, Qin [1 ]
Tao, Yongzhen [3 ]
Zhang, Hongfeng [2 ]
Zhang, Lina [3 ]
Ding, Kan [1 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China
[2] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China
[3] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2008年 / 74卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Pueraria lobata; (1 -> 6)-alpha-D-glucan; chain conformation; antioxidant effect; sulfated polysaccharide;
D O I：
10.1016/j.carbpol.2008.04.034
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A water soluble glucan, PLB-2C, was isolated from the water extract of the root of Pueraria lobota (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC) gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra, and nuclear magnetic resonance (NMR) spectroscopy of heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The results indicated that PLB-2C was a linear glucan composed of (1 -> 6)-alpha-D-Glcp. Chain conformation study showed that the polysaccharide took random coil compact conformation. In vitro cell viability assay by MTT method, its sulfated derivative PLB-2CS which was substituted at 2-0, 3-0, 4-0 positions, at 0.1, 1, and 5 mg/ml, could attenuate PC12 cell damage significantly caused by hydrogen peroxide. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：771 / 778
页数：8
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