Phenotypic characteristics of Streptococcus iniae and Streptococcus parauberis isolated from olive flounder (Paralichthys olivaceus)

被引：75

作者：

Nho, Seong-Won ^{[1
]}

Shin, Gee-Wook ^{[2
,3
]}

Park, Seong-Bin ^{[1
]}

Jang, Ho-Bin ^{[1
]}

Cha, In-Seok ^{[1
]}

Ha, Mi-Ae ^{[1
]}

Kim, Young-Rim ^{[1
]}

Park, Yon-Kyoung ^{[1
]}

Dalvi, Rishikesh S. ^{[1
]}

Kang, Bong-Jo ^{[4
]}

Joh, Seong-Joon ^{[5
]}

Jung, Tae-Sung ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Coll Vet Med, Life Sci Res Inst, Lab Aquat Anim Dis, Jinju 660701, Gyeongnam, South Korea

[2] Chonbuk Natl Univ, Coll Vet Med, Jeonju, Jeonbuk, South Korea

[3] Chonbuk Natl Univ, Biosafety Res Inst, Jeonju, Jeonbuk, South Korea

[4] Jeju Special Self Governing Prov, Fisheries Resources Res Inst, Cheju, South Korea

[5] Natl Vet Res & Quarantine Serv, Lab Aquat Anim Dis, Gyeonggi Do, South Korea

来源：

FEMS MICROBIOLOGY LETTERS | 2009年 / 293卷 / 01期

关键词：

Streptococcus parauberis; Streptococcus iniae; Paralichthys olivaceus; phenotypic characteristics; TROUT ONCORHYNCHUS-MYKISS; RAINBOW-TROUT; LACTOCOCCUS-GARVIEAE; INFECTION; FISH; CLASSIFICATION; IDENTIFICATION; CULTURE; AGENT; PCR;

D O I：

10.1111/j.1574-6968.2009.01491.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The etiological agents of streptococcosis were isolated from diseased olive flounder collected on the Jeju island of Korea. A total of 151 bacterial isolates were collected between 2003 and 2006. The isolates were examined using various phenotypic and proteomic analyses, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting, and glycoprotein assays. In addition, isolates were grown on blood agar to assess hemolytic activity, and biochemical assays were performed using the API20 Strep kit. Our results revealed that all isolates were nonmotile, Gram-positive cocci that displayed negative catalase and oxidase activities. Multiplex PCR assays revealed that 43% and 57% of the isolates were Streptococcus iniae and Streptococcus parauberis, respectively. These results were consistent with those of the SDS-PAGE and immunoblot analyses using whole-cell lysates of bacterial isolates. Significant differences were observed with respect to the Voges-Proskauer, pyrrodonyl arylamidase, alkaline phosphatase, and hemolytic activities of the S. iniae and S. parauberis isolates. Isolates of S. iniae displayed uniform profiles in the immunoblot and glycoprotein assays; however, immunoblot assays of S. parauberis isolates (using a chicken IgY antibody raised against a homologous isolate) revealed three distinct antigenic profiles. Our findings suggest that S. parauberis and S. iniae are endemic pathogens responsible for the development of streptococcosis in olive flounder.

引用

页码：20 / 27

页数：8

共 29 条

[1] Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island [J].