Bioproduction of Food Additives Hexanal and Hexanoic Acid in a Microreactor

被引：23

作者：

Salic, Anita ^{[1
]}

Pindric, Katarina ^{[1
]}

Zelic, Bruno ^{[1
]}

机构：

[1] Univ Zagreb, Fac Chem Engn & Technol, Zagreb 10000, Croatia

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2013年 / 171卷 / 08期

关键词：

Food additives; Hexanol oxidation; Hexanal; Hexanoic acid; Biocatalysis; Microreactor; COENZYME REGENERATION; SOYBEAN LIPOXYGENASE; IMMOBILIZATION; OXIDATION;

D O I：

10.1007/s12010-013-0495-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hexanal and hexanoic acid have number of applications in food and cosmetic industry because of their organoleptic characteristics. Problems like low yields, formation of unwanted by-products, and large quantities of waste in their traditional production processes are the reasons for developing new production methods. Biotransformation in a microreactor, as an alternative to classical synthesis processes, is being investigated. Because conditions in microreactors can be precisely controlled, the quality of the product and its purity can also be improved. Biocatalytic oxidation of hexanol to hexanal and hexanoic acid using suspended and immobilized permeabilized whole baker's yeast cells and suspended and immobilized purified alcohol dehydrogenase (ADH) was investigated in this study. Three different methods for covalent immobilization of biocatalyst were analyzed, and the best method for biocatalyst attachment on microchannel wall was used in the production of hexanal and hexanoic acid.

引用

页码：2273 / 2284

页数：12

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