Assembly-disassembly driven "off-on'' fluorescent perylene bisimide probes for detecting and tracking of proteins in living cells

被引：23
作者：
Bo, Fuli
Gao, Baoxiang [1 ]
Duan, Wenfeng
Li, Hongxia
Liu, Hongmei
Bai, Qianqian
机构：
[1] Hebei Univ, Key Lab Med Chem & Mol Diag, Minist Educ, Baoding 071002, Peoples R China
来源：
RSC ADVANCES | 2013年 / 3卷 / 38期
基金：
中国国家自然科学基金;
关键词：
MOLECULAR BEACONS; DYES; BIOLOGY;
D O I：
10.1039/c3ra42284d
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We have developed an assembly-disassembly driven "off-on'' fluorescent bioprobe based on amphiphilic perylene bisimide. In aqueous solutions, the amphiphilic probes can form self-assembled nanostructures with quenched fluorescence. These probes could then be disassembled by the binding of the probes to the targeted proteins, and the disassembly process leads to a 23-fold enhancement in fluorescence intensity. Amphiphilic perylene bisimides could be used as probes for the detection and tracking of targeted proteins under live cell conditions.
引用
收藏
页码：17007 / 17010
页数：4
相关论文
共 23 条
[1]   Self-assembled π-stacks of functional dyes in solution: structural and thermodynamic features [J].
Chen, Zhijian ;
Lohr, Andreas ;
Saha-Moeller, Chantu R. ;
Wuerthner, Frank .
CHEMICAL SOCIETY REVIEWS, 2009, 38 (02) :564-584
[2]   VERY SOLUBLE AND PHOTOSTABLE PERYLENE FLUORESCENT DYES [J].
DEMMIG, S ;
LANGHALS, H .
CHEMISCHE BERICHTE-RECUEIL, 1988, 121 (02) :225-230
[3]   Energy Transfer Pathways in a Rylene-Based Triad [J].
Fron, Eduard ;
Puhl, Larissa ;
Oesterling, Ingo ;
Li, Chen ;
Muellen, Klaus ;
De Schryver, Frans C. ;
Hofkens, Johan ;
Vosch, Tom .
CHEMPHYSCHEM, 2011, 12 (03) :595-608
[4]   Protease sensors for bioimaging [J].
Funovics, M ;
Weissleder, R ;
Tung, CH .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 377 (06) :956-963
[5]   Helical columnar liquid crystals based on dendritic peptides substituted perylene bisimides [J].
Gao, Baoxiang ;
Xia, Defang ;
Zhang, Licui ;
Bai, Qianqian ;
Bai, Libin ;
Yang, Tao ;
Ba, Xinwu .
JOURNAL OF MATERIALS CHEMISTRY, 2011, 21 (40) :15975-15980
[6]   Water-soluble and fluorescent dendritic perylene bisimides for live-cell imaging [J].
Gao, Baoxiang ;
Li, Hongxia ;
Liu, Hongmei ;
Zhang, Licui ;
Bai, Qianqian ;
Ba, Xinwu .
CHEMICAL COMMUNICATIONS, 2011, 47 (13) :3894-3896
[7]   Highly fluorescent water-soluble polyglycerol-dendronized perylene bisimide dyes [J].
Heek, Timm ;
Fasting, Carlo ;
Rest, Christina ;
Zhang, Xin ;
Wuerthner, Frank ;
Haag, Rainer .
CHEMICAL COMMUNICATIONS, 2010, 46 (11) :1884-1886
[8]   Terrylenimides: New NIR fluorescent dyes [J].
Holtrup, FO ;
Muller, GRJ ;
Quante, H ;
Defeyter, S ;
DeSchryver, FC ;
Mullen, K .
CHEMISTRY-A EUROPEAN JOURNAL, 1997, 3 (02) :219-225
[9]   Photophysics of New Water-Soluble Terrylenediimide Derivatives and Applications in Biology [J].
Jung, Christophe ;
Ruthardt, Nadia ;
Lewis, Robert ;
Michaelis, Jens ;
Sodeik, Beate ;
Nolde, Fabian ;
Peneva, Kalina ;
Muellen, Klaus ;
Braeuchle, Christoph .
CHEMPHYSCHEM, 2009, 10 (01) :180-190
[10]   New Strategies for Fluorescent Probe Design in Medical Diagnostic Imaging [J].
Kobayashi, Hisataka ;
Ogawa, Mikako ;
Alford, Raphael ;
Choyke, Peter L. ;
Urano, Yasuteru .
CHEMICAL REVIEWS, 2010, 110 (05) :2620-2640
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