Characterization of a novel human protein phosphatase 2C family member, PP2Cκ

被引：0

作者：

Dai, Jianfeng ^{[1
]}

Zhang, Jiyuan ^{[1
]}

Sun, Yaqiong ^{[1
]}

Wu, Qihan ^{[1
]}

Sun, Liyun ^{[1
]}

Ji, Chaoneng ^{[1
]}

Gu, Shaohua ^{[1
]}

Feng, Congjing ^{[1
]}

Xie, Yi ^{[1
]}

Mao, Yumin ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2006年 / 17卷 / 06期

关键词：

PP2C kappa; phosphotase activity; HSF signal pathway; subcellular localization;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A novel member of human protein phosphatase 2C gene named PP2C kappa was isolated from a human fetal brain cDNA library. The 2.0 kb cDNA encodes a 372 amino acid polypeptide with an intact protein phosphatase 2C (PP2C) catalytic domain. Reverse transcription-PCR (RT-PCR) revealed that the PP2C kappa was widely expressed in normal human tissues. Transient transfection suggested that PP2C kappa was localized in the nucleus in AD293 cells. Recombinant Trx-His-PP2C kappa showed phosphatase activity toward p-nitrophenyl phosphate (pNPP), as well as oligopeptides containing phospho-threonine residues. Furthermore, the overexpression of PP2C kappa distinctly activated the heat shock transcription factor pathway in eukaryotic cells.

引用

页码：1117 / 1123

页数：7

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