Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

被引：73

作者：

Johansson, Linda C. ^{[1
]}

Arnlund, David ^{[1
]}

Katona, Gergely ^{[1
]}

White, Thomas A. ^{[2
]}

Barty, Anton ^{[2
]}

DePonte, Daniel P. ^{[2
]}

Shoeman, Robert L. ^{[3
,4
]}

Wickstrand, Cecilia ^{[1
]}

Sharma, Amit ^{[1
]}

Williams, Garth J. ^{[5
]}

Aquila, Andrew ^{[2
]}

Bogan, Michael J. ^{[6
]}

Caleman, Carl ^{[2
]}

Davidsson, Jan ^{[7
]}

Doak, R. Bruce ^{[8
]}

Frank, Matthias ^{[9
]}

Fromme, Raimund ^{[10
]}

Galli, Lorenzo ^{[2
,11
]}

Grotjohann, Ingo ^{[10
]}

Hunter, Mark S. ^{[10
]}

Kassemeyer, Stephan ^{[3
,4
]}

Kirian, Richard A. ^{[8
]}

Kupitz, Christopher ^{[10
]}

Liang, Mengning ^{[2
]}

Lomb, Lukas ^{[3
,4
]}

Malmerberg, Erik ^{[1
]}

Martin, Andrew V. ^{[2
]}

Messerschmidt, Marc ^{[5
]}

Nass, Karol ^{[2
,11
]}

Redecke, Lars ^{[12
,13
]}

Seibert, M. Marvin ^{[5
]}

Sjoehamn, Jennie ^{[1
]}

Steinbrener, Jan ^{[3
,4
]}

Stellato, Francesco ^{[2
]}

Wang, Dingjie ^{[8
]}

Wahlgren, Weixaio Y. ^{[1
]}

Weierstall, Uwe ^{[8
]}

Westenhoff, Sebastian ^{[1
]}

Zatsepin, Nadia A. ^{[8
]}

Boutet, Sebastien ^{[5
]}

Spence, John C. H. ^{[8
]}

Schlichting, Ilme ^{[3
,4
]}

Chapman, Henry N. ^{[2
,11
]}

Fromme, Petra ^{[10
]}

Neutze, Richard ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, S-40530 Gothenburg, Sweden

[2] DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany

[3] Max Planck Inst Med Res, D-69120 Heidelberg, Germany

[4] Ctr Free Electron Laser Sci, Max Planck Adv Study Grp, D-22607 Hamburg, Germany

[5] SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA

[6] SLAC Natl Accelerator Lab, PULSE Inst, Menlo Pk, CA 94025 USA

[7] Uppsala Univ, Dept Chem, Angstrom Lab, S-75120 Uppsala, Sweden

[8] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

[9] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA

[10] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

[11] Univ Hamburg, Dept Phys, D-22761 Hamburg, Germany

[12] Univ Hamburg, Inst Biochem & Mol Biol, Joint Lab Struct Biol Infect & Inflammat, D-22607 Hamburg, Germany

[13] Univ Lubeck, DESY, Inst Biochem, D-22607 Hamburg, Germany

来源：

NATURE COMMUNICATIONS | 2013年 / 4卷

基金：

美国国家科学基金会;

关键词：

PROTEIN-STRUCTURE DETERMINATION; CRYSTALLIZATION;

D O I：

10.1038/ncomms3911

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 angstrom resolution and determine its serial femtosecond crystallography structure to 3.5 angstrom resolution. Although every microcrystal is exposed to a dose of 33MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

引用

页数：7

共 37 条

[31] Micro-crystallography comes of age [J].

Smith, Janet L. ;

Fischetti, Robert F. ;

Yamamoto, Masaki .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2012, 22 (05) :602-612

[32] Large-format, high-speed, X-ray pnCCDs combined with electron and ion imaging spectrometers in a multipurpose chamber for experiments at 4th generation light sources [J].

Strueder, Lothar ;

Eppa, Sascha ;

Rolles, Daniel ;

Hartmann, Robert ;

Holl, Peter ;

Lutz, Gerhard ;

Soltau, Heike ;

Eckart, Rouven ;

Reich, Christian ;

Heinzinger, Klaus ;

Thamm, Christian ;

Rudenko, Artem ;

Krasniqi, Faton ;

Kuehnel, Kai-Uwe ;

Bauer, Christian ;

Schroeter, Claus-Dieter ;

Moshammer, Robert ;

Techert, Simone ;

Miessner, Danilo ;

Porro, Matteo ;

Haelker, Olaf ;

Meidinger, Norbert ;

Kimmel, Nils ;

Andritschke, Robert ;

Schopper, Florian ;

Weidenspointner, Georg ;

Ziegler, Alexander ;

Pietschner, Daniel ;

Herrmann, Sven ;

Pietsch, Ullrich ;

Walenta, Albert ;

Leitenberger, Wolfram ;

Bostedt, Christoph ;

Moeller, Thomas ;

Rupp, Daniela ;

Adolph, Marcus ;

Graafsma, Heinz ;

Hirsemann, Helmut ;

Gaertner, Klaus ;

Richter, Rainer ;

Foucar, Lutz ;

Shoeman, Robert L. ;

Schlichting, Ilme ;

Ullrich, Joachim .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2010, 614 (03) :483-496

[33] Lipidic sponge phase crystallization of membrane proteins [J].

Wadsten, Pia ;

Wohri, Annemarie B. ;

Snijder, Arjan ;

Katona, Gergely ;

Gardiner, Alastair T. ;

Cogdell, Richard J. ;

Neutze, Richard ;

Engstrom, Sven .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 364 (01) :44-53

[34] Injector for scattering measurements on fully solvated biospecies [J].

Weierstall, U. ;

Spence, J. C. H. ;

Doak, R. B. .

REVIEW OF SCIENTIFIC INSTRUMENTS, 2012, 83 (03)

[35] CrystFEL: a software suite for snapshot serial crystallography [J].

White, Thomas A. ;

Kirian, Richard A. ;

Martin, Andrew V. ;

Aquila, Andrew ;

Nass, Karol ;

Barty, Anton ;

Chapman, Henry N. .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2012, 45 :335-341

[36] Light-Induced Structural Changes in a Photosynthetic Reaction Center Caught by Laue Diffraction [J].

Wohri, Annemarie B. ;

Katona, Gergely ;

Johansson, Linda C. ;

Fritz, Emelie ;

Malmerberg, Erik ;

Andersson, Magnus ;

Vincent, Jonathan ;

Eklund, Mattias ;

Cammarata, Marco ;

Wulff, Michael ;

Davidsson, Jan ;

Groenhof, Gerrit ;

Neutze, Richard .

SCIENCE, 2010, 328 (5978) :630-633

[37] Lipidic Sponge Phase Crystal Structure of a Photosynthetic Reaction Center Reveals Lipids on the Protein Surface [J].

Wohri, Annemarie B. ;

Wahlgren, Weixiao Y. ;

Malmerberg, Erik ;

Johansson, Linda C. ;

Neutze, Richard ;

Katona, Gergely .

BIOCHEMISTRY, 2009, 48 (41) :9831-9838

← 1 2 3 4 →