Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

被引：73

作者：

Johansson, Linda C. ^{[1
]}

Arnlund, David ^{[1
]}

Katona, Gergely ^{[1
]}

White, Thomas A. ^{[2
]}

Barty, Anton ^{[2
]}

DePonte, Daniel P. ^{[2
]}

Shoeman, Robert L. ^{[3
,4
]}

Wickstrand, Cecilia ^{[1
]}

Sharma, Amit ^{[1
]}

Williams, Garth J. ^{[5
]}

Aquila, Andrew ^{[2
]}

Bogan, Michael J. ^{[6
]}

Caleman, Carl ^{[2
]}

Davidsson, Jan ^{[7
]}

Doak, R. Bruce ^{[8
]}

Frank, Matthias ^{[9
]}

Fromme, Raimund ^{[10
]}

Galli, Lorenzo ^{[2
,11
]}

Grotjohann, Ingo ^{[10
]}

Hunter, Mark S. ^{[10
]}

Kassemeyer, Stephan ^{[3
,4
]}

Kirian, Richard A. ^{[8
]}

Kupitz, Christopher ^{[10
]}

Liang, Mengning ^{[2
]}

Lomb, Lukas ^{[3
,4
]}

Malmerberg, Erik ^{[1
]}

Martin, Andrew V. ^{[2
]}

Messerschmidt, Marc ^{[5
]}

Nass, Karol ^{[2
,11
]}

Redecke, Lars ^{[12
,13
]}

Seibert, M. Marvin ^{[5
]}

Sjoehamn, Jennie ^{[1
]}

Steinbrener, Jan ^{[3
,4
]}

Stellato, Francesco ^{[2
]}

Wang, Dingjie ^{[8
]}

Wahlgren, Weixaio Y. ^{[1
]}

Weierstall, Uwe ^{[8
]}

Westenhoff, Sebastian ^{[1
]}

Zatsepin, Nadia A. ^{[8
]}

Boutet, Sebastien ^{[5
]}

Spence, John C. H. ^{[8
]}

Schlichting, Ilme ^{[3
,4
]}

Chapman, Henry N. ^{[2
,11
]}

Fromme, Petra ^{[10
]}

Neutze, Richard ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, S-40530 Gothenburg, Sweden

[2] DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany

[3] Max Planck Inst Med Res, D-69120 Heidelberg, Germany

[4] Ctr Free Electron Laser Sci, Max Planck Adv Study Grp, D-22607 Hamburg, Germany

[5] SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA

[6] SLAC Natl Accelerator Lab, PULSE Inst, Menlo Pk, CA 94025 USA

[7] Uppsala Univ, Dept Chem, Angstrom Lab, S-75120 Uppsala, Sweden

[8] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

[9] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA

[10] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

[11] Univ Hamburg, Dept Phys, D-22761 Hamburg, Germany

[12] Univ Hamburg, Inst Biochem & Mol Biol, Joint Lab Struct Biol Infect & Inflammat, D-22607 Hamburg, Germany

[13] Univ Lubeck, DESY, Inst Biochem, D-22607 Hamburg, Germany

来源：

NATURE COMMUNICATIONS | 2013年 / 4卷

基金：

美国国家科学基金会;

关键词：

PROTEIN-STRUCTURE DETERMINATION; CRYSTALLIZATION;

D O I：

10.1038/ncomms3911

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 angstrom resolution and determine its serial femtosecond crystallography structure to 3.5 angstrom resolution. Although every microcrystal is exposed to a dose of 33MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

引用

页数：7

共 37 条

[1] PHENIX: a comprehensive Python']Python-based system for macromolecular structure solution [J].

Adams, Paul D. ;

Afonine, Pavel V. ;

Bunkoczi, Gabor ;

Chen, Vincent B. ;

Davis, Ian W. ;

Echols, Nathaniel ;

Headd, Jeffrey J. ;

Hung, Li-Wei ;

Kapral, Gary J. ;

Grosse-Kunstleve, Ralf W. ;

McCoy, Airlie J. ;

Moriarty, Nigel W. ;

Oeffner, Robert ;

Read, Randy J. ;

Richardson, David C. ;

Richardson, Jane S. ;

Terwilliger, Thomas C. ;

Zwart, Peter H. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 :213-221

[2]

Barty A, 2012, NAT PHOTONICS, V6, P35, DOI [10.1038/NPHOTON.2011.297, 10.1038/nphoton.2011.297]

[3] Overcoming barriers to membrane protein structure determination [J].

Bill, Roslyn M. ;

Henderson, Peter J. F. ;

Iwata, So ;

Kunji, Edmund R. S. ;

Michel, Hartmut ;

Neutze, Richard ;

Newstead, Simon ;

Poolman, Bert ;

Tate, Christopher G. ;

Vogel, Horst .

NATURE BIOTECHNOLOGY, 2011, 29 (04) :335-340

[4] High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography [J].

Boutet, Sebastien ;

Lomb, Lukas ;

Williams, Garth J. ;

Barends, Thomas R. M. ;

Aquila, Andrew ;

Doak, R. Bruce ;

Weierstall, Uwe ;

DePonte, Daniel P. ;

Steinbrener, Jan ;

Shoeman, Robert L. ;

Messerschmidt, Marc ;

Barty, Anton ;

White, Thomas A. ;

Kassemeyer, Stephan ;

Kirian, Richard A. ;

Seibert, M. Marvin ;

Montanez, Paul A. ;

Kenney, Chris ;

Herbst, Ryan ;

Hart, Philip ;

Pines, Jack ;

Haller, Gunther ;

Gruner, Sol M. ;

Philipp, Hugh T. ;

Tate, Mark W. ;

Hromalik, Marianne ;

Koerner, Lucas J. ;

van Bakel, Niels ;

Morse, John ;

Ghonsalves, Wilfred ;

Arnlund, David ;

Bogan, Michael J. ;

Caleman, Carl ;

Fromme, Raimund ;

Hampton, Christina Y. ;

Hunter, Mark S. ;

Johansson, Linda C. ;

Katona, Gergely ;

Kupitz, Christopher ;

Liang, Mengning ;

Martin, Andrew V. ;

Nass, Karol ;

Redecke, Lars ;

Stellato, Francesco ;

Timneanu, Nicusor ;

Wang, Dingjie ;

Zatsepin, Nadia A. ;

Schafer, Donald ;

Defever, James ;

Neutze, Richard .

SCIENCE, 2012, 337 (6092) :362-364

[5] The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) [J].

Boutet, Sebastien ;

Williams, Garth J. .

NEW JOURNAL OF PHYSICS, 2010, 12

[6] AMO instrumentation for the LCLS X-ray FEL [J].

Bozek, J. D. .

EUROPEAN PHYSICAL JOURNAL-SPECIAL TOPICS, 2009, 169 :129-132

[7] Membrane Protein Structure Determination Using Crystallography and Lipidic Mesophases: Recent Advances and Successes [J].

Caffrey, Martin ;

Li, Dianfan ;

Dukkipati, Abhiram .

BIOCHEMISTRY, 2012, 51 (32) :6266-6288

[8] On the Feasibility of Nanocrystal Imaging Using Intense and Ultrashort X-ray Pulses [J].

Caleman, Carl ;

Huldt, Gosta ;

Maia, Filipe R. N. C. ;

Ortiz, Carlos ;

Parak, Fritz G. ;

Hajdu, Janos ;

van der Spoel, David ;

Chapman, Henry N. ;

Timneanu, Nicusor .

ACS NANO, 2011, 5 (01) :139-146

[9] Femtosecond X-ray protein nanocrystallography [J].

Chapman, Henry N. ;

Fromme, Petra ;

Barty, Anton ;

White, Thomas A. ;

Kirian, Richard A. ;

Aquila, Andrew ;

Hunter, Mark S. ;

Schulz, Joachim ;

DePonte, Daniel P. ;

Weierstall, Uwe ;

Doak, R. Bruce ;

Maia, Filipe R. N. C. ;

Martin, Andrew V. ;

Schlichting, Ilme ;

Lomb, Lukas ;

Coppola, Nicola ;

Shoeman, Robert L. ;

Epp, Sascha W. ;

Hartmann, Robert ;

Rolles, Daniel ;

Rudenko, Artem ;

Foucar, Lutz ;

Kimmel, Nils ;

Weidenspointner, Georg ;

Holl, Peter ;

Liang, Mengning ;

Barthelmess, Miriam ;

Caleman, Carl ;

Boutet, Sebastien ;

Bogan, Michael J. ;

Krzywinski, Jacek ;

Bostedt, Christoph ;

Bajt, Sasa ;

Gumprecht, Lars ;

Rudek, Benedikt ;

Erk, Benjamin ;

Schmidt, Carlo ;

Hoemke, Andre ;

Reich, Christian ;

Pietschner, Daniel ;

Strueder, Lothar ;

Hauser, Guenter ;

Gorke, Hubert ;

Ullrich, Joachim ;

Herrmann, Sven ;

Schaller, Gerhard ;

Schopper, Florian ;

Soltau, Heike ;

Kuehnel, Kai-Uwe ;

Messerschmidt, Marc .

NATURE, 2011, 470 (7332) :73-U81

[10] MolProbity: all-atom structure validation for macromolecular crystallography [J].

Chen, Vincent B. ;

Arendall, W. Bryan, III ;

Headd, Jeffrey J. ;

Keedy, Daniel A. ;

Immormino, Robert M. ;

Kapral, Gary J. ;

Murray, Laura W. ;

Richardson, Jane S. ;

Richardson, David C. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 :12-21

← 1 2 3 4 →