Expression and characterization of the hepatitis E virus ORF3 protein in the methylotrophic yeast, Pichia pastoris

被引：22
作者：
Lal, SK [1 ]
Tulasiram, P [1 ]
Jameel, S [1 ]
机构：
[1] INT CTR GENET ENGN & BIOTECHNOL,VIROL GRP,NEW DELHI 110067,INDIA
来源：
GENE | 1997年 / 190卷 / 01期
关键词：
alcohol oxidase promoter; expression plasmid; integrative transformation; open reading frame; heterologous gene expression; methanol utilization pathway; glycosylation pattern; PUTATIVE STRUCTURAL PROTEINS; HIGH-LEVEL EXPRESSION; ANTIBODY-RESPONSES; GENE; CLONING; HEV;
D O I：
10.1016/S0378-1119(96)00698-1
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We have used the methylotrophic yeast, Pichia pastoris, to express the open reading frame 3 (ORF3) of the hepatitis E virus (HEV). The ORF3 gene codes for a 123-amino-acid protein that contains highly immunodominant epitopes and is a potentially useful diagnostic and immunoprophylactic antigen. The expressed protein showed positive on immunoblots probed against antibodies raised in rabbit and infected human patient sera. In order to optimize the ORF3 protein expression, we have examined the regulated expression of this protein and characterized it. Unlike its expression in E. coli, the ORF3 protein was present in both the soluble and insoluble fractions of the cell lysate. The expressed protein is not glycosylated and does not undergo any major processing in the host strain.
引用
收藏
页码：63 / 67
页数：5
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