Combined fluorescence-Raman spectroscopic setup for the diagnosis of melanocytic lesions

被引：46

作者：

Cicchi, Riccardo ^{[1
,2
]}

Cosci, Alessandro ^{[2
]}

Rossari, Susanna ^{[3
]}

Kapsokalyvas, Dimitrios ^{[2
]}

Baria, Enrico ^{[4
]}

Maio, Vincenza ^{[5
]}

Massi, Daniela ^{[5
]}

De Giorgi, Vincenzo ^{[3
]}

Pimpinelli, Nicola ^{[3
]}

Pavone, Francesco Saverio ^{[1
,2
,4
,6
]}

机构：

[1] Natl Res Council INO CNR, Natl Inst Opt, I-50125 Florence, Italy

[2] European Lab Nonlinear Spect LENS, I-50019 Sesto Fiorentino, Italy

[3] Univ Florence, Dept Crit Care Med & Surg, Div Clin Prevent & Oncol Dermatol, I-50129 Florence, Italy

[4] Univ Florence, Dept Phys, I-50019 Sesto Fiorentino, Italy

[5] Univ Florence, Dept Crit Care Med & Surg, Div Pathol, I-50134 Florence, Italy

[6] Int Ctr Computat Neurophoton ICON, I-50019 Florence, Italy

来源：

JOURNAL OF BIOPHOTONICS | 2014年 / 7卷 / 1-2期

关键词：

dermatology; fluorescence spectroscopy; Raman spectroscopy; IN-VIVO; MALIGNANT-MELANOMA; SKIN-CANCER; ABCD RULE; TISSUE; PROBES; DERMATOSCOPY; MICROSCOPY; DERMOSCOPY; DYSPLASIA;

D O I：

10.1002/jbio.201200230

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two optical fibre-based probes for spectroscopic measurements on human tissues were designed and developed. The two probes combine fluorescence and Raman spectroscopy in a multimodal approach. The fluorescence excitation was provided by two laser diodes emitting in the UV (378 nm) and in the visible (445 nm) range, while a third source in the NIR (785 nm) was used for Raman. The device was tested on freshly excised human skin biopsies clinically diagnosed as malignant melanoma, melanocytic nevus, or healthy skin. Discrimination of lesions based on their fluorescence and Raman spectra showed good correlation with the subsequent histological examination. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

引用

页码：86 / 95

页数：10

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