Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides

被引：17

作者：

Schwaighofer, Andreas ^{[1
]}

Steininger, Christoph ^{[1
]}

Hildenbrandt, David M. ^{[1
]}

Srajer, Johannes ^{[1
,2
]}

Nowak, Christoph ^{[1
,2
]}

Knoll, Wolfgang ^{[1
]}

Naumann, Renate L. C. ^{[1
]}

机构：

[1] Austrian Inst Technol, Vienna, Austria

[2] Ctr Electrochem Surface Technol, Wiener Neustadt, Austria

来源：

BIOPHYSICAL JOURNAL | 2013年 / 105卷 / 12期

关键词：

ACTIVE-SITE; PARACOCCUS-DENITRIFICANS; INFRARED-SPECTROSCOPY; HIS-TYR; HEME; MEMBRANE; PROTEIN; FTIR; SPECTROELECTROCHEMISTRY; BINDING;

D O I：

10.1016/j.bpj.2013.10.037

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.

引用

页码：2706 / 2713

页数：8

共 46 条

[11] The protein-tethered lipid bilayer: A novel mimic of the biological membrane
Giess, F
Friedrich, MG
Heberle, J
Naumann, RL
Knoll, W
[J]. BIOPHYSICAL JOURNAL, 2004, 87 (05) : 3213 - 3220
[12] SECONDARY STRUCTURE AND DOSAGE OF SOLUBLE AND MEMBRANE-PROTEINS BY ATTENUATED TOTAL REFLECTION FOURIER-TRANSFORM INFRARED-SPECTROSCOPY ON HYDRATED FILMS
GOORMAGHTIGH, E
CABIAUX, V
RUYSSCHAERT, JM
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 193 (02): : 409 - 420
[13] Redox titration of all electron carriers of cytochrome c oxidase by Fourier transform infrared spectroscopy
Gorbikova, EA
Vuorilehto, K
Wikström, M
Verkhovsky, MI
[J]. BIOCHEMISTRY, 2006, 45 (17) : 5641 - 5649
[14] Time-resolved ATR-FTIR spectroscopy of the oxygen reaction in the D124N mutant of cytochrome C oxidase from Paracoccus denitrificans
Gorbikova, Elena A.
Belevich, Nikolai P.
Wikstrom, Marten
Verkhovsky, Michael I.
[J]. BIOCHEMISTRY, 2007, 46 (45) : 13141 - 13148
[15] Time-resolved surface-enhanced resonance Raman spectro-electrochemistry of heme proteins
Grosserueschkamp, Marc
Nowak, Christoph
Knoll, Wolfgang
Naumann, Renate L. C.
[J]. SPECTROSCOPY-AN INTERNATIONAL JOURNAL, 2010, 24 (1-2): : 125 - 129
[16] DRAM: Efficient adaptive MCMC
Haario, Heikki
Laine, Marko
Mira, Antonietta
Saksman, Eero
[J]. STATISTICS AND COMPUTING, 2006, 16 (04) : 339 - 354
[17] Transient binding of CO to CuB in cytochrome c oxidase is dynamically linked to structural changes around a carboxyl group:: A time-resolved step-scan Fourier transform infrared investigation
Heitbrink, D
Sigurdson, H
Bolwien, C
Brzezinski, P
Heberle, J
[J]. BIOPHYSICAL JOURNAL, 2002, 82 (01) : 1 - 10
[18] Electrochemical and ultraviolet/visible/infrared spectroscopic analysis of heme a and a3 redox reactions in the cytochrome c oxidase from Paracoccus denitrificans:: Separation of heme a and a3 contributions and assignment of vibrational modes
Hellwig, P
Grzybek, S
Behr, J
Ludwig, B
Michel, H
Mäntele, W
[J]. BIOCHEMISTRY, 1999, 38 (06) : 1685 - 1694
[19] C-terminal truncation and histidine-tagging of cytochrome c oxidase subunit II reveals the native processing site, shows involvement of the C-terminus in cytochrome c binding, and improves the assay for proton pumping
Hiser, C
Mills, DA
Schall, M
Ferguson-Miller, S
[J]. BIOCHEMISTRY, 2001, 40 (06) : 1606 - 1615
[20] Time-resolved step-scan Fourier transform infrared investigation of heme-copper oxidases:: implications for O2 input and H2O/H+ output channels
Koutsoupakis, C
Pinakoulaki, E
Stavrakis, S
Daskalakis, V
Varotsis, C
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2004, 1655 (1-3): : 347 - 352

← 1 2 3 4 5 →