Evaluation of the enantioselectivity of carbon nanoparticles-modified chiral separation systems using dextrin as chiral selector by capillary electrokinetic chromatography

被引:24
作者
Hua, Xiaoyi [1 ]
Du, Yingxiang [1 ,2 ,3 ]
Chen, Jiaquan [1 ]
Xu, Guangfu [1 ]
Yu, Tao [1 ]
Zhang, Qi [1 ]
机构
[1] China Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
[2] China Pharmaceut Univ, Minist Educ, Key Lab Drug Qual Control & Pharmacovigilance, Nanjing 210009, Jiangsu, Peoples R China
[3] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Capillary EKC; Carbon nanoparticles; Dextrin; Enantioseparation; Pseudo-stationary phase; MODIFIED GOLD NANOPARTICLES; BASIC DRUGS; ENANTIOMERIC SEPARATION; PSEUDOSTATIONARY PHASE; CARBOGENIC NANOPARTICLES; STATIONARY-PHASE; CLINDAMYCIN PHOSPHATE; ZONE-ELECTROPHORESIS; NANOTUBES; ELECTROCHROMATOGRAPHY;
D O I
10.1002/elps.201200627
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Nanoparticles (NPs) can be used as pseudostationary phases (PSPs) in EKC, which is similar to the use of micelle additives as applied in MEKC. To date, the use of NPs to enhance enantiomeric separation by EKC with -CD or its derivative as chiral selector has been reported only in two papers. However, to the best of our knowledge, there has been no prior effort to use NPs for achieving enantioseparation with polysaccharides as chiral selector. This paper describes for the first time the use of carbon nanoparticles (CNPs) as PSPs to modify chiral separation system employing dextrin as chiral selector for the enantioseparations of several basic drugs in capillary EKC. Three different types of CNPs, including carbogenic nanoparticles (NPs), carboxylated single-walled carbon nanotubes, and carboxylated multiwalled carbon nanotubes, were used as running buffer additives, respectively. The potential of the PSPs and the effects of dextrin concentration, buffer pH, and buffer concentration on the enantioseparations were evaluated. Four pairs of tested enantiomers were successfully resolved in less than 15min with the resolution values in the range of 1.41-4.52 under optimized conditions. Compared to the buffer without NPs, the introduction of NPs into the buffer enhanced the separation of the enantiomers.
引用
收藏
页码:1901 / 1907
页数:7
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