Directed evolution of a synthetic RNA-protein module to create a new translational switch

被引：11

作者：

Hara, Tomoaki ^{[1
]}

Saito, Hirohide ^{[2
,3
,4
]}

Inoue, Tan ^{[1
,2
]}

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Lab Gene Biodynam, Sakyo Ku, Kyoto 6068502, Japan

[2] Japan Sci & Technol Agcy JST, Int Cooperat Res Project ICORP, Chiyoda Ku, Tokyo 1020075, Japan

[3] Kyoto Univ, Hakubi Ctr Adv Res, Sakyo Ku, Kyoto 6068502, Japan

[4] Kyoto Univ, Ctr iPS Cell Res & Applicat CiRA, Kyoto 6068507, Japan

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 37期

关键词：

MAMMALIAN-CELLS; BIOLOGY; IDENTIFICATION; FOUNDATIONS; SYSTEMS; PARTS; MOTIF;

D O I：

10.1039/c3cc38688k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A synthetic RNP-binding module was developed to produce an alternative translational switch: an in vitro selection experiment targeting a derivative of L7Ae protein (L7KK) identified a new H23 RNA aptamer that binds tightly to both L7KK and L7Ae. The switch serves as a translational OFF switch for constructing a NOR gate.

引用

页码：3833 / 3835

页数：3

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