Directed evolution of a synthetic RNA-protein module to create a new translational switch

被引：11
|
作者：
Hara, Tomoaki [1 ]
Saito, Hirohide [2 ,3 ,4 ]
Inoue, Tan [1 ,2 ]
机构：
[1] Kyoto Univ, Grad Sch Biostudies, Lab Gene Biodynam, Sakyo Ku, Kyoto 6068502, Japan
[2] Japan Sci & Technol Agcy JST, Int Cooperat Res Project ICORP, Chiyoda Ku, Tokyo 1020075, Japan
[3] Kyoto Univ, Hakubi Ctr Adv Res, Sakyo Ku, Kyoto 6068502, Japan
[4] Kyoto Univ, Ctr iPS Cell Res & Applicat CiRA, Kyoto 6068507, Japan
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 37期
关键词：
MAMMALIAN-CELLS; BIOLOGY; IDENTIFICATION; FOUNDATIONS; SYSTEMS; PARTS; MOTIF;
D O I：
10.1039/c3cc38688k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A synthetic RNP-binding module was developed to produce an alternative translational switch: an in vitro selection experiment targeting a derivative of L7Ae protein (L7KK) identified a new H23 RNA aptamer that binds tightly to both L7KK and L7Ae. The switch serves as a translational OFF switch for constructing a NOR gate.
引用
收藏
页码：3833 / 3835
页数：3
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