A polyethylenimine-mimetic biodegradable polycation gene vector and the effect of amine composition in transfection efficiency

被引：37

作者：

Shen, J. ^{[1
]}

Zhao, D. J. ^{[1
]}

Li, W. ^{[1
]}

Hu, Q. L. ^{[1
]}

Wang, Q. W. ^{[1
]}

Xu, F. J. ^{[2
]}

Tang, G. P. ^{[1
]}

机构：

[1] Hangzhou Univ, Inst Chem Biol & Pharmaceut Chem, Hangzhou 310028, Peoples R China

[2] Beijing Univ Chem Technol, Coll Mat Sci & Engn, Minist Educ, Key Lab Carbon Fiber & Funct Polymers, Beijing 100029, Peoples R China

来源：

BIOMATERIALS | 2013年 / 34卷 / 18期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

Gene delivery; Vector; Polyethylenimine; Biodegradable; Mimic; MOLECULAR-WEIGHT; IN-VITRO; DNA DELIVERY; THERAPY; DRUG; VIVO; CYTOTOXICITY; MICELLES; POLYMERS; CARRIER;

D O I：

10.1016/j.biomaterials.2013.02.068

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The low toxicity and efficient gene delivery of polymeric vectors remain the major barrier to the clinical application of non-viral gene therapy. Here, we present a poly-D, c-succinimide (PSI)-based biodegradable cationic polymer which mimicked the golden standard, branched polyethylenimine (PEI, similar to 25 kDa). To investigate the influence of 1 degrees, 2 degrees, 3 degrees amine group ratio in the polymer, a series of PSI-based vectors (PSI-NNx'-NNy) grafted with different amine side chains of N,N-dimethyldipropylenetriamine (NN') and bis(3-aminopropyl)amine (NN) were first characterized and contrasted by biophysical measurements. The in vitro and in vivo biological assay demonstrated that PSI-NN0.85'-NN1 exhibited better transfection ability and biocompatibility than PEI. The present results suggest that such PEI-mimic biodegradable PSI-NN0.85'-NN1 possesses a good potential application for clinical gene delivery. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：4520 / 4531

页数：12

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