Real-Time PCR Detection of gyrA and parC Mutations in Streptococcus pneumoniae

被引：6

作者：

Page, S. ^{[1
,2
]}

Vernel-Pauillac, F. ^{[2
]}

O'Connor, O. ^{[1
]}

Bremont, S. ^{[3
]}

Charavay, F. ^{[1
]}

Courvalin, P. ^{[3
]}

Goarant, C.

Le Hello, S. ^{[1
]}

机构：

[1] Inst Pasteur Nouvelle Caledonie, Lab Epidemiol Mol, Noumea 98845, New Caledonia

[2] Lab Rech Bacteriol, Noumea, New Caledonia

[3] Inst Pasteur, Unite Agents Antibacteriens, F-75724 Paris, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2008年 / 52卷 / 11期

关键词：

D O I：

10.1128/AAC.00082-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.

引用

页码：4155 / 4158

页数：4

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