Isoflurane post-treatment improves pulmonary vascular permeability via upregulation of heme oxygenase-1

被引:9
作者
Dong, Xiang [1 ]
Hu, Rong [1 ]
Sun, Yu [1 ]
Li, Qifang [1 ]
Jiang, Hong [1 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Anesthesiol, Shanghai 200011, Peoples R China
基金
中国国家自然科学基金;
关键词
heme oxygenase-1; induced nitric oxide synthase; isoflurane; post-treatment; pulmonary vascular permeability; NITRIC-OXIDE SYNTHASE; ACUTE LUNG INJURY; CARBON-MONOXIDE; MURINE SEPSIS; ENDOTOXIN; RATS; EXPRESSION; LIPOPOLYSACCHARIDE; MACROPHAGES; INHIBITOR;
D O I
10.3109/01902148.2013.817627
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Isoflurane (ISO) has been shown to attenuate acute lung injury (ALI). Induction of heme oxygenase-1 (HO-1) and suppression of inducible nitric oxide synthase (iNOS) expression provide cytoprotection in lung and vascular injury. The aim of this study was to investigate the effect of post-treatment with isoflurane on lung vascular permeability and the role of HO-1 in an ALI rat model induced by cecal ligation and puncture (CLP). Male Sprague-Dawley rats were randomly assigned to one of four groups: sham group, sham rats post-treated with vehicle (Sham); CLP group, CLP rats post-treated with vehicle (CLP); ISO group, CLP rats post-treated with isoflurane (ISO); and ZnPP group, CLP rats injected with zinc protoporphyrin IX (ZnPP), a competitive inhibitor of HO-1, 1 hour before the operation, and post-treated with isoflurane (ZnPP). Isoflurane (1.4%) was administered 2 hour after CLP. At 24 hour after CLP, the extent of ALI was evaluated by lung wet/dry ratio, Evans blue dye (EBD) extravasation, lung permeability index (LPI), as well as histological and immunohistochemical examinations. We also determined pulmonary iNOS and HO-1 expression. Compared with the CLP group, the isoflurane post-treatment group showed improved pulmonary microvascular permeability as detected by EBD extravasation, LPI, as well as histological and immunohistochemical examinations. Furthermore, isoflurane decreased iNOS and increased HO-1 expression in lung tissue. Pretreatment with ZnPP prevented the protective effects of isoflurane in rats. These findings indicate that the protective role of isoflurane post-conditioning against CLP-induced lung injury may be associated with its role in upregulating HO-1 in ALI.
引用
收藏
页码:295 / 303
页数:9
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