Separation of M-like current and ERG current in NG108-15 cells

1 Differentiated NG108-15 neuroblastoma x glioma hybrid cells were whole-cell voltage-clamped. Hyperpolarizing pulses, superimposed on a depolarized holding potential(-30 or -20 mV), elicited deactivation currents which consisted of two components, distinguishable by fitting with two exponential functions. 2 Linopirdine [DuP 996, 3,3-bis(4-pyridinylmethyl)- 1-phenylindolin-2-one], a neurotransmitter-release enhancer known as potent and selective blocker of the M-current of rat sympathetic neurons, in concentrations of 5 or 10 mu M selectively inhibited the fast component (IC50 = 14.7 mu M). The slow component was less sensitive to linopirdine (IC50 > 20 mu M). 3 The class III antiarrhythmics [(4-methylsulphonyl)amido]benzenesulphonamide (WAY-123.398) and 1-[2-(6-methyl-2-pyrydinil)ethyl]-4-(4-methylsulphonylaminobenzoyl) piperidine (E-4031), selective inhibitors of the inwardly rectifying ERG (ether-ri-go-go-related gene) potassium channel, inhibited predominantly the slow component (IC50 = 38 nM for E-4031). The time constant of the WAY-123.398-sensitive current resembled the time constant of the slow component in size and voltage dependence. 4 Inwardly rectifying ERG currents, recorded in K+-rich bath at strongly negative pulse potentials, resembled the slow component of the deactivation current in their low sensitivity to linopirdine (28% inhibition at 50 mu M). 5 The size of the slow component varied greatly between cells. Accordingly, varied the effect of WAY-123.398 on deactivation current and holding current. 6 RNA transcripts for the following members of the ether-ri-go-go gene (EAG) K+ channel family were found in differentiated NG108-15 cells: ERG1, ERG2, EAG1, EAG-like (ELK)1, ELK2; ERG3 was only present in non-differentiated cells. In addition, RNA transcripts for KCNQ2 and KCNQ3 were found in differentiated and non-differentiated cells. 7 We conclude that the fast component of the deactivation current is M-like current and the slow component is deactivating ERG current. The molecular correlates are probably KCNQ2/KCNQ3 and ERG1/ERG2, respectively.