STRmix™ collaborative exercise on DNA mixture interpretation

被引：32

作者：

Bright, Jo-Anne ^{[1
]}

Cheng, Kevin ^{[1
]}

Kerr, Zane ^{[1
]}

McGovern, Catherine ^{[1
]}

Kelly, Hannah ^{[1
]}

Moretti, Tamyra R. ^{[2
]}

Smith, Michael A. ^{[2
]}

Bieber, Frederick R. ^{[3
,4
]}

Budowle, Bruce ^{[5
]}

Coble, Michael D. ^{[5
]}

Alghafrr, Rashed ^{[6
]}

Allen, Paul Stafford ^{[7
]}

Barbee, Amy ^{[8
]}

Beamer, Amy Barbee Vickie ^{[9
]}

Buettner, Christina ^{[10
]}

Russell, Melanie ^{[11
]}

Gehrig, Christian ^{[12
]}

Hicks, Tacha ^{[13
]}

Charak, Jessica ^{[14
]}

Cheong-Wing, Kate ^{[15
]}

Ciecko, Anne ^{[16
]}

Davis, Christie T. ^{[17
]}

Donley, Michael ^{[18
]}

Pedersen, Natalie ^{[19
]}

Gartside, Bill ^{[20
]}

Granger, Dominic ^{[21
]}

Greer-Ritzheimer, Marymargaret ^{[22
]}

Reisinger, Erick ^{[23
]}

Kennedy, Jarrah ^{[24
]}

Grammer, Erin ^{[25
]}

Kaplan, Marla ^{[26
]}

Hansen, David ^{[27
]}

Larsen, Hans J. ^{[28
]}

Laureano, Alanna ^{[29
]}

Li, Christina ^{[30
]}

Lien, Eugene ^{[31
]}

Lindberg, Emilia ^{[32
]}

Kelly, Ciara ^{[33
]}

Mallinder, Ben ^{[34
]}

Malsom, Simon ^{[35
]}

Yacovone-Margetts, Alyse ^{[36
]}

McWhorter, Andrew ^{[37
]}

Prajapati, Sapana M. ^{[38
]}

Powell, Tamar ^{[39
]}

Shutler, Gary ^{[40
]}

Stevenson, Kate ^{[1
]}

Stonehouse, April R. ^{[41
]}

Smith, Lindsey ^{[42
]}

Murakami, Julie ^{[43
]}

Halsing, Eric ^{[44
]}

机构：

[1] Inst Environm Sci & Res Ltd, Private Bag 92021, Auckland 1142, New Zealand

[2] Fed Bur Invest Lab, DNA Support Unit, 2501 Invest Pkwy, Quantico, VA 22135 USA

[3] Brigham & Womens Hosp, Ctr Adv Mol Diagnost, Dept Pathol, 75 Francis St, Boston, MA 02115 USA

[4] Harvard Med Sch, 75 Francis St, Boston, MA 02115 USA

[5] Univ North Texas, Ctr Human Identificat, Dept Microbiol Immunol & Genet, Hlth Sci Ctr, 3500 Camp Bowie Blvd, Ft Worth, TX 76107 USA

[6] Dubai Police GHQ, Gen Dept Forens Sci & Criminol, Dubai, U Arab Emirates

[7] Cellmark Forens Serv, Abingdon, Oxon, England

[8] Massachusetts State Police Crime Lab, Sudbury, MA USA

[9] Scottsdale Police Dept Crime Lab, Scottsdale, AZ USA

[10] Wyoming State Crime Lab, Cheyenne, WY USA

[11] CT DESPP Div Sci Serv, Middletown, CT USA

[12] Univ Ctr Legal Med, CURML, Lausanne, Switzerland

[13] Univ Lausanne, Sch Criminal Justice, Lausanne, Switzerland

[14] Las Vegas Metropolitan Police Dept, Las Vegas, NV USA

[15] Northern Terr Police Fire & Emergency Serv, Darwin, NT, Australia

[16] Midwest Reg Forens Lab, Minneapolis, MN USA

[17] Helix Analyt Inc, Hawthorne, NY USA

[18] Harris Cty Inst Forens Sci, Houston, TX USA

[19] Victoria Police Forens Sci Lab, Macleod, Vic, Australia

[20] San Bernardino Cty Sheriffs Dept, San Bernardino, CA USA

[21] Lab Sci Judiciaires & Med Legale, Montreal, PQ, Canada

[22] DuPage Cty Sheriffs Crime Lab, Wheaton, IL USA

[23] Orange Cty Crime Lab, Santa Ana, CA USA

[24] Kansas City Police Crime Lab, Kansas City, MO USA

[25] Indiana State Police Lab, Indianapolis, IN USA

[26] Oregon State Police Portland Metro Crime Lab, Portland, OR USA

[27] Forens Analyt Crime Lab, Hayward, CA USA

[28] Univ Copenhagen, Copenhagen, Denmark

[29] Westchester Cty Dept Labs & Res, Valhalla, NY USA

[30] Govt Lab, Hong Kong, Peoples R China

[31] New York City Off Chief Med Examiner OCME, New York, NY USA

[32] Forens Lab Finland, NBI, Vantaa, Finland

[33] Forens Sci Ireland, Dublin, Ireland

[34] Scottish Police Author, Glasgow, Lanark, Scotland

[35] Key Forens Serv, Coventry, W Midlands, England

[36] Palm Beach Cty Sheriffs Off, W Palm Beach, FL USA

[37] Texas Dept Publ Safety, Austin, TX USA

[38] Signat Sci LLC, Austin, TX USA

[39] San Francisco Police Dept, Crime Lab, San Francisco, CA USA

[40] Washington State Patrol, Olympia, WA USA

[41] Mesa Police Dept Forens Serv, Mesa, AZ USA

[42] US Army Criminal Invest Lab, Arsenal, AL USA

[43] PathWest, Nedlands, WA, Australia

[44] Calif Dept Justice, Sacramento, CA USA

[45] Idaho State Police Forens Serv, Pocatello, ID USA

[46] Florida Dept Law Enforcement, Tallahassee, FL USA

[47] Forens Sci SA, GPO Box 2790, Adelaide, SA 5001, Australia

[48] Flinders Univ S Australia, Sch Biol Sci, GPO Box 2100, Adelaide, SA 5001, Australia

[49] Univ Auckland, Dept Stat, Auckland, New Zealand

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2019年 / 40卷

关键词：

Forensic DNA interpretation; Probabilistic genotyping; STRmix; Inter-laboratory study; Intra-laboratory study; INTERNAL VALIDATION; PROFILES; SINGLE; NUMBER; MIX13;

D O I：

10.1016/j.fsigen.2019.01.006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An intra and inter-laboratory study using the probabilistic genotyping (PG) software STRmix (TM) is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems (TM) 3500 Series Genetic Analyzer, were selected. 174 participants responded. For Sample 1 (low template, in the order of 200 rfu for major contributors) five participants described the comparison as inconclusive with respect to the POI or excluded him. Where LRs were assigned, the point estimates ranging from 2 x 10(4) to 8 x 10(6). For Sample 2 (in the order of 2000 rfu for major contributors), LRs ranged from 2 x 10(28) to 2 x 10(29). Where LRs were calculated, the differences between participants can be attributed to (from largest to smallest impact): varying number of contributors (NoC), the exclusion of some loci within the interpretation, differences in local CE data analysis methods leading to variation in the peaks present and their heights in the input files used, and run-to-run variation due to the random sampling inherent to all MCMC-based methods. This study demonstrates a high level of repeatability and reproducibility among the participants. For those results that differed from the mode, the differences in LR were almost always minor or conservative.

引用

页码：1 / 8

页数：8

共 19 条

[1] A large-scale dataset of single and mixed-source short tandem repeat profiles to inform human identification strategies: PROVEDIt [J].

Alfonse, Lauren E. ;

Garrett, Amanda D. ;

Lun, Desmond S. ;

Duffy, Ken R. ;

Grgicak, Catherine M. .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2018, 32 :62-70

[2]

[Anonymous], 2015, GeneMapper ID-X Software Version 1.0.1/1.1 Reference Guide

[3] GHEP-ISFG collaborative exercise on mixture profiles (GHEP-MIX06). Reporting conclusions: Results and evaluation [J].

Barrio, P. A. ;

Crespillo, M. ;

Luque, J. A. ;

Aler, M. ;

Baeza-Richer, C. ;

Baldassarri, L. ;

Carnevali, E. ;

Coufalova, P. ;

Flores, I ;

Garcia, O. ;

Garcia, M. A. ;

Gonzalez, R. ;

Hernandez, A. ;

Ingles, V ;

Luque, G. M. ;

Mosquera-Miguel, A. ;

Pedrosa, S. ;

Pontes, M. L. ;

Porto, M. J. ;

Posada, Y. ;

Ramella, M., I ;

Ribeiro, T. ;

Riego, E. ;

Sala, A. ;

Saragoni, V. G. ;

Serrano, A. ;

Vannelli, S. .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2018, 35 :156-163

[4] Internal validation of STRmix™ - A multi laboratory response to PCAST [J].

Bright, Jo-Anne ;

Richards, Rebecca ;

Kruijver, Maarten ;

Kelly, Hannah ;

McGovern, Catherine ;

Magee, Alan ;

McWhorter, Andrew ;

Ciecko, Anne ;

Peck, Brian ;

Baumgartner, Chase ;

Buettner, Christina ;

McWilliams, Scott ;

McKenna, Claire ;

Gallacher, Colin ;

Mallinder, Ben ;

Wright, Darren ;

Johnson, Deven ;

Catella, Dorothy ;

Lien, Eugene ;

O'Connor, Craig ;

Duncan, George ;

Bundy, Jason ;

Echard, Jillian ;

Lowe, John ;

Stewart, Joshua ;

Corrado, Kathleen ;

Gentile, Sheila ;

Kaplan, Marla ;

Hassler, Michelle ;

McDonald, Naomi ;

Hulme, Paul ;

Oefelein, Rachel H. ;

Montpetit, Shawn ;

Strong, Melissa ;

Noel, Sarah ;

Malsom, Simon ;

Myers, Steven ;

Welti, Susan ;

Moretti, Tamyra ;

McMahon, Teresa ;

Grill, Thomas ;

Kalafut, Tim ;

Greer-Ritzheimer, MaryMargaret ;

Beamer, Vickie ;

Taylor, Duncan A. ;

Buckleton, John S. .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2018, 34 :11-24

[5] Developmental validation of STRmix™, expert software for the interpretation of forensic DNA profiles [J].

Bright, Jo-Anne ;

Taylor, Duncan ;

McGovern, Catherine ;

Cooper, Stuart ;

Russell, Laura ;

Abarno, Damien ;

Buckleton, John .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2016, 23 :226-239

[6] The variability in likelihood ratios due to different mechanisms [J].

Bright, Jo-Anne ;

Stevenson, Kate E. ;

Curran, James M. ;

Buckleton, John S. .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2015, 14 :187-190

[7] A series of recommended tests when validating probabilistic DNA profile interpretation software [J].

Bright, Jo-Anne ;

Evett, Ian W. ;

Taylor, Duncan ;

Curran, James M. ;

Buckleton, John .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2015, 14 :125-131

[8] The effect of the uncertainty in the number of contributors to mixed DNA profiles on profile interpretation [J].

Bright, Jo-Anne ;

Curran, James M. ;

Buckleton, John S. .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2014, 12 :208-214

[9] Searching mixed DNA profiles directly against profile databases [J].

Bright, Jo-Anne ;

Taylor, Duncan ;

Curran, James ;

Buckleton, John .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2014, 9 :102-110

[10] NIST interlaboratory studies involving DNA mixtures (MIX13): A modern analysis [J].

Buckleton, John S. ;

Bright, Jo-Anne ;

Cheng, Kevin ;

Budowle, Bruce ;

Coble, Michael D. .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2018, 37 :172-179

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