Versatile design and synthesis platform for visualizing genomes with Oligopaint FISH probes

被引：300

作者：

Beliveau, Brian J. ^{[1
]}

Joyce, Eric F. ^{[1
]}

Apostolopoulos, Nicholas ^{[1
]}

Yilmaz, Feyza ^{[1
]}

Fonseka, Chamith Y. ^{[1
]}

McCole, Ruth B. ^{[1
]}

Chang, Yiming ^{[1
]}

Li, Jin Billy ^{[1
]}

Senaratne, Tharanga Niroshini ^{[1
]}

Williams, Benjamin R. ^{[1
]}

Rouillard, Jean-Marie ^{[2
,3
]}

Wu, Chao-ting ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[2] MYcroarray, Ann Arbor, MI 48105 USA

[3] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 52期

基金：

美国国家卫生研究院;

关键词：

IN-SITU HYBRIDIZATION; PEPTIDE NUCLEIC-ACIDS; OLIGONUCLEOTIDE PROBES; HUMAN-CHROMOSOMES; GENE DETECTION; MESSENGER-RNA; DNA; ORGANIZATION; LOCALIZATION; MICROSCOPY;

D O I：

10.1073/pnas.1213818110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A host of observations demonstrating the relationship between nuclear architecture and processes such as gene expression have led to a number of new technologies for interrogating chromosome positioning. Whereas some of these technologies reconstruct intermolecular interactions, others have enhanced our ability to visualize chromosomes in situ. Here, we describe an oligonucleotide- and PCR-based strategy for fluorescence in situ hybridization (FISH) and a bioinformatic platform that enables this technology to be extended to any organism whose genome has been sequenced. The oligonucleotide probes are renewable, highly efficient, and able to robustly label chromosomes in cell culture, fixed tissues, and metaphase spreads. Our method gives researchers precise control over the sequences they target and allows for single and multicolor imaging of regions ranging from tens of kilobases to megabases with the same basic protocol. We anticipate this technology will lead to an enhanced ability to visualize interphase and metaphase chromosomes.

引用

页码：21301 / 21306

页数：6

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