Versatile design and synthesis platform for visualizing genomes with Oligopaint FISH probes

被引：301

作者：

Beliveau, Brian J. ^{[1
]}

Joyce, Eric F. ^{[1
]}

Apostolopoulos, Nicholas ^{[1
]}

Yilmaz, Feyza ^{[1
]}

Fonseka, Chamith Y. ^{[1
]}

McCole, Ruth B. ^{[1
]}

Chang, Yiming ^{[1
]}

Li, Jin Billy ^{[1
]}

Senaratne, Tharanga Niroshini ^{[1
]}

Williams, Benjamin R. ^{[1
]}

Rouillard, Jean-Marie ^{[2
,3
]}

Wu, Chao-ting ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[2] MYcroarray, Ann Arbor, MI 48105 USA

[3] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 52期

基金：

美国国家卫生研究院;

关键词：

IN-SITU HYBRIDIZATION; PEPTIDE NUCLEIC-ACIDS; OLIGONUCLEOTIDE PROBES; HUMAN-CHROMOSOMES; GENE DETECTION; MESSENGER-RNA; DNA; ORGANIZATION; LOCALIZATION; MICROSCOPY;

D O I：

10.1073/pnas.1213818110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A host of observations demonstrating the relationship between nuclear architecture and processes such as gene expression have led to a number of new technologies for interrogating chromosome positioning. Whereas some of these technologies reconstruct intermolecular interactions, others have enhanced our ability to visualize chromosomes in situ. Here, we describe an oligonucleotide- and PCR-based strategy for fluorescence in situ hybridization (FISH) and a bioinformatic platform that enables this technology to be extended to any organism whose genome has been sequenced. The oligonucleotide probes are renewable, highly efficient, and able to robustly label chromosomes in cell culture, fixed tissues, and metaphase spreads. Our method gives researchers precise control over the sequences they target and allows for single and multicolor imaging of regions ranging from tens of kilobases to megabases with the same basic protocol. We anticipate this technology will lead to an enhanced ability to visualize interphase and metaphase chromosomes.

引用

页码：21301 / 21306

页数：6

共 53 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, SF
Madden, TL
Schaffer, AA
Zhang, JH
Zhang, Z
Miller, W
Lipman, DJ
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
[2] A NEW METHOD FOR FLUORESCENCE MICROSCOPICAL LOCALIZATION OF SPECIFIC DNA-SEQUENCES BY INSITU HYBRIDIZATION OF FLUOROCHROME-LABELED RNA
BAUMAN, JGJ
WIEGANT, J
BORST, P
VANDUIJN, P
[J]. EXPERIMENTAL CELL RESEARCH, 1980, 128 (02) : 485 - 490
[3] Bienko M, 2013, NAT METHODS, V10, P122, DOI [10.1038/NMETH.2306, 10.1038/nmeth.2306]
[4] Fluorescence in situ hybridization with high-complexity repeat-free oligonucleotide probes generated by massively parallel synthesis
Boyle, Shelagh
Rodesch, Matthew J.
Halvensleben, Heather A.
Jeddeloh, Jeffrey A.
Bickmore, Wendy A.
[J]. CHROMOSOME RESEARCH, 2011, 19 (07) : 901 - 909
[5] Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development
Briones, Carlos
Moreno, Miguel
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2012, 402 (10) : 3071 - 3089
[6] A GENE FROM THE REGION OF THE HUMAN X-INACTIVATION CENTER IS EXPRESSED EXCLUSIVELY FROM THE INACTIVE X-CHROMOSOME
BROWN, CJ
BALLABIO, A
RUPERT, JL
LAFRENIERE, RG
GROMPE, M
TONLORENZI, R
WILLARD, HF
[J]. NATURE, 1991, 349 (6304) : 38 - 44
[7] Chaumeil Julie, 2008, V463, P297, DOI 10.1007/978-1-59745-406-3_18
[8] Chromosome Territories
Cremer, Thomas
Cremer, Marion
[J]. COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2010, 2 (03): : a003889
[9] A decade of 3C technologies: insights into nuclear organization
de Wit, Elzo
de laat, Wouter
[J]. GENES & DEVELOPMENT, 2012, 26 (01) : 11 - 24
[10] Capturing chromosome conformation
Dekker, J
Rippe, K
Dekker, M
Kleckner, N
[J]. SCIENCE, 2002, 295 (5558) : 1306 - 1311

← 1 2 3 4 5 6 →