Comparative Study of the Photoprotolytic Reactions of D-Luciferin and Oxyluciferin

被引：42

作者：

Erez, Yuval ^{[1
]}

Presiado, Itay ^{[1
]}

Gepshtein, Rinat ^{[1
]}

da Silva, Luis Pinto ^{[2
]}

Esteves da Silva, Joaquim C. G. ^{[2
]}

Huppert, Dan ^{[1
]}

机构：

[1] Tel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Tel Aviv, Israel

[2] Univ Porto, Fac Ciencias, Dept Quim, Ctr Invest Quim, P-4169007 Oporto, Portugal

来源：

JOURNAL OF PHYSICAL CHEMISTRY A | 2012年 / 116卷 / 28期

基金：

以色列科学基金会;

关键词：

INTERMOLECULAR PROTON-TRANSFER; FIREFLY LUCIFERASE; PHOTINUS-PYRALIS; BIOLUMINESCENCE; COLOR; LIGHT; PH; MODULATION; MECHANISM; DYNAMICS;

D O I：

10.1021/jp301910p

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Optical steady-state and time-resolved spectroscopic methods were used to study the photoprotolytic reaction of oxyluciferin, the active bioluminescence chromophore of the firefly's luciferase-catalyzed reaction. We found that like D-luciferin, the substrate of the firefly bioluminescence reaction, oxyluciferin is a photoacid with pK(a)* value of similar to 0.5, whereas the excited-state proton transfer (ESPT) rate coefficient is 2.2 x 10(10) s(-1), which is somewhat slower than that of D-luciferin. The kinetic isotope effect (KIE) on the fluorescence decay of oxyluciferin is 2.5 +/- 0.1, the same value as that of D-luciferin. Both chromophores undergo fluorescence quenching in solutions with a pH value below 3.

引用

页码：7452 / 7461

页数：10

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