Gallic acid inhibits migration and invasion in human osteosarcoma U-2 OS cells through suppressing the matrix metalloproteinase-2/-9, protein kinase B (PKB) and PKC signaling pathways

被引:108
|
作者
Liao, Ching-Lung [1 ]
Lai, Kuang-Chi [2 ,3 ]
Huang, An-Cheng [4 ]
Yang, Jai-Sing [5 ]
Lin, Jen-Jyh [1 ,6 ]
Wu, Shin-Hwar [7 ]
Wood, W. Gibson [8 ]
Lin, Jaung-Geng [1 ]
Chung, Jing-Gung [9 ,10 ]
机构
[1] China Med Univ, Grad Inst Chinese Med, Taichung 404, Taiwan
[2] China Med Univ, Sch Med, Taichung 404, Taiwan
[3] China Med Univ, Dept Surg, Beigang Hosp, Yunlin 651, Taiwan
[4] St Marys Med Nursing & Management Coll, Dept Nursing, Yilan 266, Taiwan
[5] China Med Univ, Dept Pharmacol, Taichung 404, Taiwan
[6] China Med Univ Hosp, Div Cardiol, Taichung 404, Taiwan
[7] Changhua Christian Hosp, Dept Internal Med, Div Crit Care Med, Changhua 500, Taiwan
[8] Univ Minnesota, Dept Pharmacol, VA Med Ctr, Sch Med,Geriatr Research Educ & Clin Ctr, Minneapolis, MN 55455 USA
[9] China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan
[10] Asia Univ, Dept Biotechnol, Taichung 413, Taiwan
关键词
Gallic acid; Migration and invasion; Human osteosarcoma U-2 OS cells; MMP-2/-9; AKT/PKB; PKC; ENDOPLASMIC-RETICULUM STRESS; CANCER CELLS; PHOSPHORYLATION SITES; MOLECULAR-CLONING; INDUCED APOPTOSIS; CYCLE ARREST; METASTASIS; IDENTIFICATION; EXPRESSION; AND-9;
D O I
10.1016/j.fct.2012.02.033
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Advanced cancer is a multifactorial disease which complicates treatment if the cancer cells have metastasized calling for the targeting of multiple cellular pathways. Gallic acid (GA) is known to possess multiple pharmacological activity including antitumor effects. This study investigated the mechanisms for the anticancer properties of GA on migration and invasion of human osteosarcoma U-2 OS cells. The migration and invasion in U-2 OS cells were determined by a Boyden chamber transwell assay. The expression levels and activities of MMP-2 and MMP-9 were measured by Western blotting, real-time PCR and gelatin zymography assays. All examined proteins levels from Western blotting indicated that GA decreased the protein levels of GRB2, PI3K, AKT/PKB, PKC, p38, ERK1/2, JNK, NF-kappa B p65 in U-2 OS cells. GA also inhibited the activities of AICT, 1KK and PKC by in vitro kinase assay. GA suppressed the migration and invasive ability of U-2 OS cells, and it decreased MMP-2 and MMP-9 protein and mRNA levels and secreted enzyme activities in vitro. These results suggest that potential signaling pathways of GA-inhibited migration and invasion in U-2 OS cells may be due to down-regulation of PKC, inhibition of mitogen-activated protein kinase (MAPK) and PI3K/AKT, resulting in inhibition of MMP-2 and MMP-9 expressions. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1734 / 1740
页数:7
相关论文
共 50 条
  • [41] Ursonic acid inhibits migration and invasion of human osteosarcoma cells through the suppression of mitogen-activated protein kinases and matrix metalloproteinases
    Son, Juhyeon
    Cha, Hansol
    Lee, Sungeun
    Bae, Yongwoong
    Ryou, Chongsuk
    Lee, Sang Yeol
    MOLECULAR BIOLOGY REPORTS, 2023, 50 (05) : 4029 - 4038
  • [42] Ursonic acid inhibits migration and invasion of human osteosarcoma cells through the suppression of mitogen-activated protein kinases and matrix metalloproteinases
    Juhyeon Son
    Hansol Cha
    Sungeun Lee
    Yongwoong Bae
    Chongsuk Ryou
    Sang Yeol Lee
    Molecular Biology Reports, 2023, 50 : 4029 - 4038
  • [43] Crude extract of Euphorbia formosana inhibits the migration and invasion of DU145 human prostate cancer cells: The role of matrix metalloproteinase-2/9 inhibition via the MAPK signaling pathway
    Yang, Jiun-Long
    Lin, Ju-Hwa
    Weng, Shu-Wen
    Chen, Jaw-Chyun
    Yang, Jai-Sing
    Amagaya, Sakae
    Funayana, Shinji
    Wood, W. Gibson
    Kuo, Chao-Lin
    Chung, Jing-Gung
    MOLECULAR MEDICINE REPORTS, 2013, 7 (05) : 1403 - 1408
  • [44] Galectin-9 Promotes Human Trophoblast Cell Invasion through Matrix Metalloproteinase-2 and p38 Signaling Pathway
    Sun FengRun
    Chen ChunQing
    Yu Min
    Wang SongCun
    Li DaJin
    Du MeiRong
    生殖与发育医学(英文), 2018, 2 (01) : 1 - 7
  • [45] Galectin-9 Promotes Human Trophoblast Cell Invasion through Matrix Metalloproteinase-2 and p38 Signaling Pathway
    Sun, Feng-Run
    Chen, Chun-Qing
    Yu, Min
    Wang, Song-Cun
    Li, Da-Jin
    Du, Mei-Rong
    REPRODUCTIVE AND DEVELOPMENTAL MEDICINE, 2018, 2 (01) : 1 - +
  • [46] Wingless (Wnt)-3A Induces Trophoblast Migration and Matrix Metalloproteinase-2 Secretion through Canonical Wnt Signaling and Protein Kinase B/AKT Activation
    Sonderegger, Stefan
    Haslinger, Peter
    Sabri, Alia
    Leisser, Christina
    Otten, Jan V.
    Fiala, Christian
    Knoefler, Martin
    ENDOCRINOLOGY, 2010, 151 (01) : 211 - 220
  • [47] Perfluorooctanoic Acid Enhances Invasion of Follicular Thyroid Carcinoma Cells Through NF-κB and Matrix Metalloproteinase-2 Activation
    Saejia, Pipop
    Lirdprapamongkol, Kriengsak
    Svasti, Jisnuson
    Paricharttanakul, N. Monique
    ANTICANCER RESEARCH, 2019, 39 (05) : 2429 - 2435
  • [48] Scutellarin inhibits the growth and invasion of human tongue squamous carcinoma through the inhibition of matrix metalloproteinase-2 and-9 and αvβ6 integrin
    Li, Haixia
    Huang, Dayong
    Gao, Zhongxiuzi
    Chen, Yan
    Zhang, Lina
    Zheng, Jinhua
    INTERNATIONAL JOURNAL OF ONCOLOGY, 2013, 42 (05) : 1674 - 1681
  • [49] Sinulariolide Suppresses Cell Migration and Invasion by Inhibiting Matrix Metalloproteinase-2/-9 and Urokinase through the PI3K/AKT/mTOR Signaling Pathway in Human Bladder Cancer Cells
    Cheng, Te-Chih
    Din, Zhong-Hao
    Su, Jui-Hsin
    Wu, Yu-Jen
    Liu, Chih-I
    MARINE DRUGS, 2017, 15 (08)
  • [50] Tricetin suppresses the migration/invasion of human glioblastoma multiforme cells by inhibiting matrix metalloproteinase-2 through modulation of the expression and transcriptional activity of specificity protein 1
    Chao, Rockey
    Chow, Jyh-Ming
    Hsieh, Yi-Hsien
    Chen, Chi-Kuan
    Lee, Wei-Jiunn
    Hsieh, Feng-Koo
    Yu, Nuo-Yi
    Chou, Ming-Chih
    Cheng, Chao-Wen
    Yang, Shun-Fa
    Chien, Ming-Hsien
    EXPERT OPINION ON THERAPEUTIC TARGETS, 2015, 19 (10) : 1293 - 1306