Combination of aptamer with gold nanoparticles for electrochemical signal amplification: Application to sensitive detection of platelet-derived growth factor

被引：122
作者：
Wang, Jing [1 ,2 ]
Meng, Wenying [1 ,2 ]
Zheng, Xiaofeng [1 ,2 ]
Liu, Shanli [3 ]
Li, Genxi [1 ,2 ]
机构：
[1] Nanjing Univ, Natl Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
[3] Shanghai Univ, Sch Life Sci, Lab Biosensing Technol, Shanghai 200444, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Electrochemistry; Aptamer; Gold nanoparticles; Platelet-derived growth factor; ROLLING CIRCLE AMPLIFICATION; LABEL-FREE; DNA; REAGENTLESS; APTASENSOR; THROMBIN; PLATFORM; BINDING; SURFACE;
D O I：
10.1016/j.bios.2008.08.030
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this paper, we report a novel electrochemical detection approach for platelet-derived growth factor (PDGF) via "sandwich" structure and gold nanoparticles (Au-NPs) mediated amplification technique. The "sandwich" structure is fabricated based on the fact that PDGF has two aptamer-binding sites, which makes it possible for one PDGF molecule to connect with two aptamers simultaneously. It is found that this electrochemical system with "sandwich" structure and Au-NPs can significantly amplify the signal of electrochemical probe (Ru(NH3)(5)Cl](2+) for PDGF detection, and thus increase the detection sensitivity significantly. As a result, this PDGF detection approach obtains an extraordinarily low detection limit of 1 x 10(-14) M for purified samples, 1 x 10(-12) M for contaminated-ridden samples or undiluted blood serum. This detection approach can also exhibit good stability and excellent specificity. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：1598 / 1602
页数：5
相关论文
共 35 条
[21]   Electrochemical quantitation of DNA immobilized on gold [J].
Steel, AB ;
Herne, TM ;
Tarlov, MJ .
ANALYTICAL CHEMISTRY, 1998, 70 (22) :4670-4677
[22]   One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes [J].
Storhoff, JJ ;
Elghanian, R ;
Mucic, RC ;
Mirkin, CA ;
Letsinger, RL .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (09) :1959-1964
[23]   Ultrasensitive electrochemical immunosensor for clinical immunoassay using thionine-doped magnetic gold nanospheres as labels and horseradish peroxidase as enhancer [J].
Tang, Dianping ;
Yuan, Ruo ;
Chal, Yaqin .
ANALYTICAL CHEMISTRY, 2008, 80 (05) :1582-1588
[24]   SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT - RNA LIGANDS TO BACTERIOPHAGE-T4 DNA-POLYMERASE [J].
TUERK, C ;
GOLD, L .
SCIENCE, 1990, 249 (4968) :505-510
[25]   Investigation of molecular beacon aptamer-based bioassay for platelet-derived growth factor detection [J].
Vicens, MC ;
Sen, A ;
Vanderlaan, A ;
Drake, TJ ;
Tan, WH .
CHEMBIOCHEM, 2005, 6 (05) :900-907
[26]   A gold nanoparticle-based aptamer target binding readout for ATP assay [J].
Wang, Jing ;
Wang, Lihua ;
Liu, Xingfen ;
Liang, Zhiqiang ;
Song, Shiping ;
Li, Wenxin ;
Li, Genxi ;
Fan, Chunhai .
ADVANCED MATERIALS, 2007, 19 (22) :3943-3946
[27]   Simple and sensitive aptamer-based colorimetric sensing of protein using unmodified gold nanoparticle probes [J].
Wei, Hui ;
Li, Bingling ;
Li, Jing ;
Wang, Erkang ;
Dong, Shaojun .
CHEMICAL COMMUNICATIONS, 2007, (36) :3735-3737
[28]   Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor [J].
Xiao, Y ;
Lubin, AA ;
Heeger, AJ ;
Plaxco, KW .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (34) :5456-5459
[29]   Real-time rolling circle amplification for protein detection [J].
Yang, Litao ;
Fung, Christine W. ;
Cho, Eun Jeong ;
Ellington, Andrew D. .
ANALYTICAL CHEMISTRY, 2007, 79 (09) :3320-3329
[30]   Label-free and reagentless aptamer-based sensors for small molecules [J].
Zayats, Maya ;
Huang, Ye ;
Gill, Ron ;
Ma, Chun-an ;
Willner, Itamar .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (42) :13666-13667
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