Combination of aptamer with gold nanoparticles for electrochemical signal amplification: Application to sensitive detection of platelet-derived growth factor

被引：122
作者：
Wang, Jing [1 ,2 ]
Meng, Wenying [1 ,2 ]
Zheng, Xiaofeng [1 ,2 ]
Liu, Shanli [3 ]
Li, Genxi [1 ,2 ]
机构：
[1] Nanjing Univ, Natl Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
[3] Shanghai Univ, Sch Life Sci, Lab Biosensing Technol, Shanghai 200444, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Electrochemistry; Aptamer; Gold nanoparticles; Platelet-derived growth factor; ROLLING CIRCLE AMPLIFICATION; LABEL-FREE; DNA; REAGENTLESS; APTASENSOR; THROMBIN; PLATFORM; BINDING; SURFACE;
D O I：
10.1016/j.bios.2008.08.030
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this paper, we report a novel electrochemical detection approach for platelet-derived growth factor (PDGF) via "sandwich" structure and gold nanoparticles (Au-NPs) mediated amplification technique. The "sandwich" structure is fabricated based on the fact that PDGF has two aptamer-binding sites, which makes it possible for one PDGF molecule to connect with two aptamers simultaneously. It is found that this electrochemical system with "sandwich" structure and Au-NPs can significantly amplify the signal of electrochemical probe (Ru(NH3)(5)Cl](2+) for PDGF detection, and thus increase the detection sensitivity significantly. As a result, this PDGF detection approach obtains an extraordinarily low detection limit of 1 x 10(-14) M for purified samples, 1 x 10(-12) M for contaminated-ridden samples or undiluted blood serum. This detection approach can also exhibit good stability and excellent specificity. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：1598 / 1602
页数：5
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