Crystal Structure of a Bioactive Pactamycin Analog Bound to the 30S Ribosomal Subunit

被引：17

作者：

Tourigny, David S. ^{[1
]}

Fernandez, Israel S. ^{[1
]}

Kelley, Ann C. ^{[1
]}

Vakiti, Ramkrishna Reddy ^{[2
]}

Chattopadhyay, Amit Kumar ^{[2
]}

Dorich, Stephane ^{[2
]}

Hanessian, Stephen ^{[2
]}

Ramakrishnan, V. ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 0QH, England

[2] Univ Montreal, Dept Chem, Stn Ctr Vile, Montreal, PQ H3C 3J7, Canada

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2013年 / 425卷 / 20期

基金：

英国医学研究理事会; 英国惠康基金; 加拿大自然科学与工程研究理事会;

关键词：

ANTIBIOTICS; ANTITUMOR; RNA; BINDING; SITES;

D O I：

10.1016/j.jmb.2013.05.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biosynthetically and chemically derived analogs of the antibiotic pactamycin and de-6-methylsalicyly1 (MSA)-pactarnycin have attracted recent interest as potential antiprotozoal and antitumor drugs. Here, we report a 3.1-angstrom crystal structure of de-6-MSA-pactamycin bound to its target site on the Thermus thermophilus 30S ribosomal subunit. Although de-6-MSA-pactamycin lacks the MSA moiety, it shares the same binding site as pactamycin and induces a displacement of nucleic acid template bound at the E-site of the 30S. The structure highlights unique interactions between this pactamycin analog and the ribosome, which paves the way for therapeutic development of related compounds. (C) 2013 The Authors. Published by Elsevier Ltd. All tights reserved.

引用

页码：3907 / 3910

页数：4

共 16 条

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