Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems

被引：1

作者：

Bicalho, Juliana Marques ^{[1
]}

Fonseca Junior, Antonio Augusto ^{[2
]}

Sousa de Oliveira, Cairo Henrique ^{[3
]}

Resende, Claudia Fideles ^{[4
]}

Kassar, Telissa da Cunha ^{[5
]}

Florentino Galinari, Grazielle Cossenzo ^{[1
]}

Oliveira, Andre Penido ^{[6
]}

Orzil, Livia de Lima ^{[7
]}

Pimenta dos Reis, Jenner Karlisson ^{[8
]}

Leite, Romulo Cerqueira ^{[8
]}

机构：

[1] Univ Fed Minas Gerais, Ciencia Anim, Belo Horizonte, MG, Brazil

[2] LANAGRO, Lab Nacl Agr, Fed Agr, Pedro Leopoldo, MG, Brazil

[3] Univ Fed Goias, Goiania, Go, Brazil

[4] Univ Fed Minas Gerais, Curso Mestrado Ciencia Anim, Belo Horizonte, MG, Brazil

[5] Univ Fed Minas Gerais, Curso Doutorado Ciencia Anim, Belo Horizonte, MG, Brazil

[6] EPAMIG, Empresa Pesquisa Agr Minas Gerais, Uberaba, MG, Brazil

[7] LANAGRO, Lab Nacl Agr, Pedro Leopoldo, MG, Brazil

[8] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil

来源：

SEMINA-CIENCIAS AGRARIAS | 2016年 / 37卷 / 05期

关键词：

Bovines; Reproduction; Uterus; BTV; BLV; SEROTYPE; 8; INFECTION; CATTLE; LYMPHOSARCOMA; TRANSMISSION; PERFORMANCE; PREVALENCE; FERTILITY; EXPOSURE; LEUKOSIS;

D O I：

10.5433/1679-0359.2016v37n5p3125

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Reproductive performance of dairy cows has a direct impact on herd productivity. Infectious agents, such as Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV), are associated with reproductive failure. However, it remains unknown if these viruses are present in the uterus and cause gestational loss. This study used molecular methods to assess if BTV and BLV can be detected in the uterus of serologically positive dairy cows with a record of abortions, stillbirths and repeat breeding (n=23) and without a record of reproductive problems (n =23). The cows came from three dairy herds of the state of Minas Gerais, Brazil. BTV was not detected in any of the uterine biopsies. Proviral DNA of BLV was detected in 54.5 % of the seropositive cows, but positivity for BLV in the uterus was not associated with the existence of reproductive problems. In conclusion, this study shows that BLV, but not BTV, is present in the uterus of seropositive cows, regardless of reproductive performance.

引用

页码：3125 / 3131

页数：7

共 38 条

[1] Transmission of bovine viral diarrhea virus (BVDV) via in vitro-fertilized embryos to recipients, but not to their offspring [J].

Bielanski, A. ;

Algire, J. ;

Lalonde, A. ;

Nadin-Davis, S. .

THERIOGENOLOGY, 2009, 71 (03) :499-508

[2]

BRASIL. Ministerio da Agricultura Pecuaria e Abastecimento, 2006, PROGR NAC CONTR ERR

[3] THE IMPLICATION OF BLV INFECTION IN THE PRODUCTIVITY, REPRODUCTIVE CAPACITY AND SURVIVAL RATE OF A DAIRY-COW [J].

BRENNER, J ;

VANHAAM, M ;

SAVIR, D ;

TRAININ, Z .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1989, 22 (03) :299-305

[4] Distribution of bluetongue virus in tissues of experimentally infected pregnant dogs as determined by in situ hybridization [J].

Brown, CC ;

Rhyan, JC ;

Grubman, MJ ;

Wilbur, LA .

VETERINARY PATHOLOGY, 1996, 33 (03) :337-340

[5] Signalment and Clinical Complaints Initiating Hospital Admission, Methods of Diagnosis, and Pathological Findings Associated with Bovine Lymphosarcoma (112 Cases) [J].

Burton, A. J. ;

Nydam, D. V. ;

Long, E. D. ;

Divers, T. J. .

JOURNAL OF VETERINARY INTERNAL MEDICINE, 2010, 24 (04) :960-964

[6] Transplacental infection in goats experimentally infected with a European strain of bluetongue virus serotype 8 [J].

Coetzee, Peter ;

Stokstad, Maria ;

Myrmel, Mette ;

Mutowembwa, Paidamwoyo ;

Loken, Torleiv ;

Venter, Estelle H. ;

Van Vuuren, Moritz .

VETERINARY JOURNAL, 2013, 197 (02) :335-341

[7] Productive and reproductive performance in cattle infected with bovine leukosis virus [J].

D'Angelino, JL ;

Garcia, M ;

Birgel, EH .

JOURNAL OF DAIRY RESEARCH, 1998, 65 (04) :693-695

[8] MILK AND FAT YIELDS DECLINE IN BOVINE LEUKEMIA VIRUS-INFECTED HOLSTEIN CATTLE WITH PERSISTENT LYMPHOCYTOSIS [J].

DA, Y ;

SHANKS, RD ;

STEWART, JA ;

LEWIN, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (14) :6538-6541

[9] EXPERIMENTAL-INFECTION OF PREGNANT CATTLE WITH BLUETONGUE VIRUS SEROTYPE-11 BETWEEN POSTBREEDING DAYS 21 AND 48 [J].

DELACONCHABERMEJILLO, A ;

ODEON, A ;

BONDURANT, RH ;

OSBURN, BI .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1993, 5 (03) :329-335

[10] Embryonic and early foetal losses in cattle and other ruminants [J].

Diskin, M. G. ;

Morris, D. G. .

REPRODUCTION IN DOMESTIC ANIMALS, 2008, 43 :260-267

← 1 2 3 4 →