Proton Gradients Produced by Glucose Oxidase Microcapsules Containing Motor F0F1-ATPase for Continuous ATP Biosynthesis

被引：42

作者：

Duan, Li ^{[1
]}

Qi, Wei ^{[1
]}

Yan, Xuehai ^{[1
]}

He, Qiang ^{[1
,2
]}

Cui, Yue ^{[1
]}

Wang, Kewei ^{[1
]}

Li, Dongxiang ^{[1
]}

Li, Junbai ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Key Lab Colloid & Interface Sci, Int Joint Lab,Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China

[2] Max Planck Inst Colloids & Interfaces, D-14476 Golm, Germany

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2009年 / 113卷 / 02期

关键词：

SYNTHASE; HOLLOW; HYDROLYSIS; LIPOSOMES; CF0F1;

D O I：

10.1021/jp807883e

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Glucose oxidase (GOD) microcapsules held together by cross-linker, glutaraldehyde (GA), are fabricated by the layer-by-layer (LbL) assembly technique. The lipid bilayer containing CF0F1-ATPase was coated on the outer shell of GOD microcapsules. Driven under the proton gradients produced by catalysis of GOD microcapsules for glucose, ATP is synthesized from ADP and inorganic phosphate catalyzed by the ATPase rotary catalysis. The results show here that ATPase reconstituted on the GOD microcapsules retains its catalytic activity.

引用

页码：395 / 399

页数：5

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