Effects of Vasoactive Intestinal Polypeptide on Proliferation and Migration of Human Buccal Mucosa Fibroblasts

被引：0
作者：
Jin, Lan [1 ]
Chen, Changhui [2 ]
Jiang, Xinjun [3 ]
机构：
[1] Zhuji Peoples Hosp Zhejiang Prov, Dept Dent, Zhuji, Zhejiang, Peoples R China
[2] Hubei Coll Chinese Med, Dept Stomatol, Jingzhou, Hubei, Peoples R China
[3] Chunan Tradit Chinese Med Hosp, Dept Stomatol, Chunan 311700, Zhejiang, Peoples R China
来源：
JOURNAL OF HARD TISSUE BIOLOGY | 2022年 / 31卷 / 02期
关键词：
Vasoactive intestinal peptide; Human buccal mucosal fibroblast; Proliferation; Migration; Oral lichen planus;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
To explore the effects of vasoactive intestinal polypeptide (VIP) on the proliferation and migration of human buccal mucosal fibroblasts (HBMFs). HBMFs were primarily cultured and pretreated with lipopolysaccharide (LPS) as an inflammatory stimulant. The cells in experimental group and control group were transfected with overexpressed pcDNA3.1-VIP and its control pcDNA3.1-VIP-NC, and LPS group was also set. All cells were routinely cultured. Then the cell proliferative activity and migration capacity were detected by MTT assay and wound healing assay, respectively. The mRNA expressions of N-cadherin and proliferating cell nuclear antigen (PCNA) were determined using qRT-PCR, and the expression levels of VIP and macrophage metalloelastase (MME) were detected by Western blotting. Compared with LPS group, the cell proliferative activity and migration capacity, the mRNA expressions of N-cadherin and PCNA, and the expressions of VIP and MME were significantly increased in experimental group (P<0.05). Overexpression of VIP can enhance the proliferation and migration of HBMFs, probably by regulating the expression of MME.
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页码：121 / 126
页数：6
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