Beyond Identification: Emerging and Future Uses for MALDI-TOF Mass Spectrometry in the Clinical Microbiology Laboratory

被引：35

作者：

DeMarco, Mari L. ^{[1
]}

Ford, Bradley A. ^{[2
]}

机构：

[1] Univ British Columbia, St Pauls Hosp, Dept Pathol & Lab Med, Vancouver, BC V6Z 1Y6, Canada

[2] Univ Iowa Hosp & Clin, Dept Pathol, Iowa City, IA 52242 USA

来源：

CLINICS IN LABORATORY MEDICINE | 2013年 / 33卷 / 03期

关键词：

Antimicrobial susceptibility testing; Bacteremia; Bloodstream infection; Direct identification; MALDI-TOF; Mass spectrometry; Urinary tract infection; Machine learning; DESORPTION IONIZATION-TIME; BLOOD-CULTURE BOTTLES; ASSISTED-LASER-DESORPTION/IONIZATION; RAPID IDENTIFICATION; STAPHYLOCOCCUS-AUREUS; URINE SAMPLES; BACTERIA; RESISTANCE; DIFFERENTIATION; BIOTYPER;

D O I：

10.1016/j.cll.2013.03.013

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The routine use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized microorganism identification in the clinical microbiology laboratory. Building from these now common microorganism identification strategies, this review explores future clinical applications of MALDI-TOF MS. This includes practical approaches for laboratorians interested in implementing direct identification processing methods for MALDI-TOF detection of microbes in bloodstream infection (BSI) and urinary tract infection (UTI), as well as, post-analytical approaches for classifying MALDI-TOF spectral data to detect characteristics other and species-level identification (e.g. strain-level classification, typing, and resistance mechanisms).

引用

页码：611 / +

页数：19

共 75 条

[1]

[Anonymous], CLIN CHEM

[2]

[Anonymous], 2011, ACM T INTEL SYST TEC, DOI DOI 10.1145/1961189.1961199

[3]

[Anonymous], 2012, PLOS ONE

[4] Machine learning methods for predictive proteomics [J].

Barla, Annalisa ;

Jurman, Giuseppe ;

Riccadonna, Samantha ;

Merler, Stefano ;

Chierici, Marco ;

Furlanello, Cesare .

BRIEFINGS IN BIOINFORMATICS, 2008, 9 (02) :119-128

[5] Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Identification of Clinically Relevant Bacteria [J].

Benagli, Cinzia ;

Rossi, Viviana ;

Dolina, Marisa ;

Tonolla, Mauro ;

Petrini, Orlando .

PLOS ONE, 2011, 6 (01)

[6] MALDI-TOF MS Andromas strategy for the routine identification of bacteria, mycobacteria, yeasts, Aspergillus spp. and positive blood cultures [J].

Bille, E. ;

Dauphin, B. ;

Leto, J. ;

Bougnoux, M. -E. ;

Beretti, J. -L. ;

Lotz, A. ;

Suarez, S. ;

Meyer, J. ;

Join-Lambert, O. ;

Descamps, P. ;

Grall, N. ;

Mory, F. ;

Dubreuil, L. ;

Berche, P. ;

Nassif, X. ;

Ferroni, A. .

CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (11) :1117-1125

[7] Comparison of the MALDI Biotyper System Using Sepsityper Specimen Processing to Routine Microbiological Methods for Identification of Bacteria from Positive Blood Culture Bottles [J].

Buchan, Blake W. ;

Riebe, Katherine M. ;

Ledeboer, Nathan A. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (02) :346-352

[8] Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Detect Carbapenem Resistance within 1 to 2.5 Hours [J].

Burckhardt, Irene ;

Zimmermann, Stefan .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (09) :3321-3324

[9] Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification [J].

Carbonnelle, Etienne ;

Grohs, Patrick ;

Jacquier, Herve ;

Day, Nesrine ;

Tenza, Sylvie ;

Dewailly, Alexandra ;

Vissouarn, Odile ;

Rottman, Martin ;

Herrmann, Jean-Louis ;

Podglajen, Isabelle ;

Raskine, Laurent .

JOURNAL OF MICROBIOLOGICAL METHODS, 2012, 89 (02) :133-136

[10] SIGNAL DETECTABILITY - THE USE OF ROC CURVES AND THEIR ANALYSES [J].

CENTOR, RM .

MEDICAL DECISION MAKING, 1991, 11 (02) :102-106

← 1 2 3 4 5 6 7 8 →