Localization of MCC (mutated in colorectal cancer) in various tissues of mice and its involvement in cell differentiation

被引:13
|
作者
Senda, T
Matsumine, A
Yanai, H
Akiyama, T
机构
[1] Nagoya Univ, Sch Med, Dept Anat 1, Showa Ku, Nagoya, Aichi 4668550, Japan
[2] Osaka Univ, Microbial Dis Res Inst, Dept Oncogene Res, Suita, Osaka 565, Japan
[3] Univ Tokyo, Inst Mol & Biol Sci, Lab Mol & Genet Informat, Tokyo, Japan
关键词
MCC; signaling pathway; cell cycle; cell differentiation; immunohistochemistry; immunoelectron microscopy;
D O I
10.1177/002215549904700907
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Localization of the MCC (mutated in colorectal cancer) gene product, a cell cycle-regulating protein mutated in several colorectal tumors, in various mouse tissues was examined by immunohistochemistry and immunoelectron microscopy. MCC was localized on microvilli and in the apical cytoplasm in renal proximal tubule epithelial cells and pancreatic acinar cells. In hepatocytes, MCC was exclusively detected on microvilli. MCC was highly expressed in the cerebral cortex and the molecular layer of the cerebellar cortex and was partially associated with membrane organelles in neuronal elements. Adrenal chromaffin cells showed little expression of MCC, MCC was localized to the cell margins of ependymal cells, thyroid follicular cells, and anterior pituitary cells. In parotid acinar cells, only the apical surface was immunopositive. MCC was not expressed in skeletal and cardiac muscle. MCC was present at lateral cell borders in the duodenum and colon epithelium. In addition, the apical cytoplasm of colon epithelial cells exhibited intense immunoreactivity. The amount of MCC increased during differentiation of NGF-treated PC12 cells. In conclusion, MCC was expressed in differentiated cells and was associated with the plasma membrane and membrane organelles. In addition to the negative regulation of the cell cycle, MCC may be involved in cell differentiation.
引用
收藏
页码:1149 / 1157
页数:9
相关论文
共 50 条
  • [21] Differential expression of neuroleukin in osseous tissues and its involvement in mineralization during osteoblast differentiation
    Zhi, J
    Sommerfeldt, DW
    Rubin, CT
    Hadjiargyrou, M
    JOURNAL OF BONE AND MINERAL RESEARCH, 2001, 16 (11) : 1994 - 2004
  • [22] The mouse Dermokine: characterization of its various isoforms and their involvement in the late steps of epidermal differentiation
    Leclerc, E. A.
    Huchenq, A.
    Gazeilles, L.
    Mallet, A.
    Serre, G.
    Jonca, N.
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2012, 132 : S75 - S75
  • [23] Oncogenic signaling is coupled to colorectal cancer cell differentiation state
    Sell, Thomas
    Klotz, Christian
    Fischer, Matthias M.
    Astaburuaga-Garcia, Rosario
    Krug, Susanne
    Drost, Jarno
    Clevers, Hans
    Sers, Christine
    Morkel, Markus
    Bluethgen, Nils
    JOURNAL OF CELL BIOLOGY, 2023, 222 (06):
  • [24] Localization of RGD-CAP and its involvement in cell adhesion.
    Ohno, S
    Kawamoto, T
    Hashimoto, K
    Yan, W
    Noshiro, M
    Kato, Y
    Tanne, K
    JOURNAL OF DENTAL RESEARCH, 1997, 76 (05) : 1173 - 1173
  • [25] Mitochondrial fusion-fission dynamics and its involvement in colorectal cancer
    Wu, Zihong
    Xiao, Chong
    Li, Fang
    Huang, Wenbo
    You, Fengming
    Li, Xueke
    MOLECULAR ONCOLOGY, 2024, 18 (05) : 1058 - 1075
  • [26] Colorectal cancer atlas: An integrative resource for genomic and proteomic annotations from colorectal cancer cell lines and tissues
    Chisanga, David
    Keerthikumar, Shivakumar
    Pathan, Mohashin
    Ariyaratne, Dinuka
    Kalra, Hina
    Boukouris, Stephanie
    Mathew, Nidhi Abraham
    Al Saffar, Haidar
    Gangoda, Lahiru
    Ang, Ching-Seng
    Sieber, Oliver M.
    Mariadason, John M.
    Dasgupta, Ramanuj
    Chilamkurti, Naveen
    Mathivanan, Suresh
    NUCLEIC ACIDS RESEARCH, 2016, 44 (D1) : D969 - D974
  • [27] Clinical impact of minimal cancer cell detection in various colorectal cancer specimens
    Kazuhiko Yoshimatsu
    Hajime Yokomizo
    Yoshihiko Naritaka
    World Journal of Gastroenterology, 2014, (35) : 12458 - 12461
  • [28] Clinical impact of minimal cancer cell detection in various colorectal cancer specimens
    Yoshimatsu, Kazuhiko
    Yokomizo, Hajime
    Naritaka, Yoshihiko
    WORLD JOURNAL OF GASTROENTEROLOGY, 2014, 20 (35) : 12458 - 12461
  • [29] Localization and osteoblastic differentiation potential of neural crest-derived cells in oral tissues of adult mice
    Ono, Miki
    Suzawa, Tetsuo
    Takami, Masamichi
    Yamamoto, Gou
    Hosono, Tomohiko
    Yamada, Atsushi
    Suzuki, Dai
    Yoshimura, Kentaro
    Watahiki, Junichi
    Hayashi, Ryuhei
    Arata, Satoru
    Mishima, Kenji
    Nishida, Kohji
    Osumi, Noriko
    Maki, Koutaro
    Kamijo, Ryutaro
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2015, 464 (04) : 1209 - 1214
  • [30] THE TISSUE LOCALIZATION OF META-AMSA AND ITS EFFECT ON THYMIDINE INCORPORATION IN VARIOUS TISSUES INVIVO
    HELLMAN, B
    ANDERSSON, BS
    SLANINA, P
    MOHAMMED, A
    BRANDT, I
    BERAN, M
    MEDICAL ONCOLOGY AND TUMOR PHARMACOTHERAPY, 1986, 3 (02): : 87 - 94