Localization of MCC (mutated in colorectal cancer) in various tissues of mice and its involvement in cell differentiation

被引:13
|
作者
Senda, T
Matsumine, A
Yanai, H
Akiyama, T
机构
[1] Nagoya Univ, Sch Med, Dept Anat 1, Showa Ku, Nagoya, Aichi 4668550, Japan
[2] Osaka Univ, Microbial Dis Res Inst, Dept Oncogene Res, Suita, Osaka 565, Japan
[3] Univ Tokyo, Inst Mol & Biol Sci, Lab Mol & Genet Informat, Tokyo, Japan
关键词
MCC; signaling pathway; cell cycle; cell differentiation; immunohistochemistry; immunoelectron microscopy;
D O I
10.1177/002215549904700907
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Localization of the MCC (mutated in colorectal cancer) gene product, a cell cycle-regulating protein mutated in several colorectal tumors, in various mouse tissues was examined by immunohistochemistry and immunoelectron microscopy. MCC was localized on microvilli and in the apical cytoplasm in renal proximal tubule epithelial cells and pancreatic acinar cells. In hepatocytes, MCC was exclusively detected on microvilli. MCC was highly expressed in the cerebral cortex and the molecular layer of the cerebellar cortex and was partially associated with membrane organelles in neuronal elements. Adrenal chromaffin cells showed little expression of MCC, MCC was localized to the cell margins of ependymal cells, thyroid follicular cells, and anterior pituitary cells. In parotid acinar cells, only the apical surface was immunopositive. MCC was not expressed in skeletal and cardiac muscle. MCC was present at lateral cell borders in the duodenum and colon epithelium. In addition, the apical cytoplasm of colon epithelial cells exhibited intense immunoreactivity. The amount of MCC increased during differentiation of NGF-treated PC12 cells. In conclusion, MCC was expressed in differentiated cells and was associated with the plasma membrane and membrane organelles. In addition to the negative regulation of the cell cycle, MCC may be involved in cell differentiation.
引用
收藏
页码:1149 / 1157
页数:9
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