Free Radical Scavenging, α-Glucosidase Inhibitory and Anti-Inflammatory Constituents from Indian Sedges, Cyperus scariosus R.Br and Cyperus rotundus L.

Background: Cyperus scariosus R. Br and Cyperus rotundus L are widely used in ayurvedic preparation for the treatment of diabetes and other diseases. The early literature, so far, does not indicate the presence of any bioactive principle isolated from these plants. Objective: To identify free radical scavenging, anti-diabetic and anti-inflammatory principles from these two species. Materials and Methods: The bioassay guided fractionation and isolation of active constituents was done by chromatographic techniques. They also evaluated their anti-oxidant activity by DPPH and ABTS. The anti-diabetic activity was screened by alpha- glucosidase and alpha- amylase assays. Also, the further evaluation of in vitro anti-inflammatory activity using THP-1 monocytic cells and in vivo anti-inflammatory activity, was confirmed by carrageenan induced rat paw edema as model. Results: The activity guided isolation led to isolation of twelve compounds Which are: Stigmasterol([1]), beta-sitosterol([2]), Lupeol([3]), Gallic acid([4]), Quercetin([5]), beta-amyrin([6]), Oleanolic acid([7]), beta-amyrin acetate([8]), 4-hydroxyl butyl cinnamate([9]), 4-hydroxyl cinnamic acid([10]), Caffeic acid,([11]) and Kaempferol([12]) respectively. Among the isolates, the compounds 4 and 5 displayed potent radical scavenging activity with an IC50 values of 0.43 and 0.067 mu g/ml. The compounds 4, 5 and 10 showed significant antidiabetic activities. while lupeol([3]) showed potent IL-1 beta activity inhibition in THP-1 monocytic cells and also displayed significant (p<0.0025) in vivo anti-inflammatory activity. Conclusion: Inbrief, we isolated twelve compounds from both the species. Collectively, our results suggested that aromatic compounds showed good anti-oxidant and anti-diabetic activities.