Cymantrene conjugation modulates the intracellular distribution and induces high cytotoxicity of a cell-penetrating peptide

被引：49

作者：

Neundorf, Ines ^{[1
]}

Hoyer, Jan ^{[1
]}

Splith, Katrin ^{[1
]}

Rennert, Robert ^{[1
]}

N'Dongo, Harmel W. Peindy ^{[2
]}

Schatzschneider, Ulrich ^{[2
]}

机构：

[1] Univ Leipzig, Inst Biochem, D-04103 Leipzig, Germany

[2] Ruhr Univ Bochum, Lehrstuhl Anorgan Chem Bioanorgan Chem 1, D-44801 Bochum, Germany

来源：

CHEMICAL COMMUNICATIONS | 2008年 / 43期

关键词：

D O I：

10.1039/b812799a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The conjugation of cymantrene CpMn(CO)(3) to cell-penetrating peptide hCT(18-32)-k7 alters the intracellular distribution in MCF-7 cells compared to the unmodified peptide, as visualized by fluorescence microscopy, and leads to an increased nuclear accumulation; the peptide and cymantrene compound themselves are not toxic, but the bioconjugate shows a significant cytotoxicity with an IC50 value of 36 mu mol l(-1).

引用

页码：5604 / 5606

页数：3

共 21 条

[1]

DABARD R, 1972, B SOC CHIM FR, P3594

[2] The use of solid-phase synthesis techniques for the preparation of peptide-metal complex conjugates [J].

Dirscherl, Georg ;

Koenig, Burkhard .

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2008, 2008 (04) :597-634

[3] Decoding the entry of two novel cell-penetrating peptides in HeLa cells: Lipid raft-mediated endocytosis and endosomal escape [J].

Foerg, C ;

Ziegler, U ;

Fernandez-Carneado, J ;

Giralt, E ;

Rennert, R ;

Beck-Sickinger, AG ;

Merkle, HP .

BIOCHEMISTRY, 2005, 44 (01) :72-81

[4]

GINZBURG AG, 1993, RUSS CHEM REV, V62, P1025

[5] Electrochemical microbead-basel immunoassay using an (η5-cyclopentadgenyl)tricarbonylmanganese redox marker bound to bovine serum albumin [J].

Hromadová, M ;

Salmain, M ;

Fischer-Durand, N ;

Pospísil, L ;

Jaouen, G .

LANGMUIR, 2006, 22 (01) :506-511

[6]

Jaouen G, 2006, BIOORGANOMETALLICS: BIOMOLECULES, LABELING, MEDICINE, P1

[7] Transduction peptides: from technology to physiology [J].

Joliot, A ;

Prochiantz, A .

NATURE CELL BIOLOGY, 2004, 6 (03) :189-196

[8]

Langel U., 2006, HDB CELL PENETRATING

[9] THE SYNTHESIS OF METALLOCENE-LABELED DRUGS FOR BIOLOGICAL ASSAYS [J].

LAVASTRE, I ;

BESANCON, J ;

BROSSIER, P ;

MOISE, C .

APPLIED ORGANOMETALLIC CHEMISTRY, 1990, 4 (01) :9-17

[10]

LEPLOUZENNEC M, 1972, B SOC CHIM FR, P3600

← 1 2 3 →