The role of interleukin-12 in the pathogenesis of psoriasis

被引：53

作者：

Shaker, OG ^{[1
]}

Moustafa, W

Essmat, S

Abdel-Halim, M

El-Komy, M

机构：

[1] Cairo Univ, Fac Med, Dept Biochem, Cairo, Egypt

[2] Cairo Univ, Dept Dermatol, Fac Med, Cairo, Egypt

来源：

CLINICAL BIOCHEMISTRY | 2006年 / 39卷 / 02期

关键词：

psoriasis; interleukin-12; interferon gamma; in situ PCR;

D O I：

10.1016/j.clinbiochem.2005.11.008

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: To verify the role of IL-12 in the pathogenesis of psoriasis and determine its relation to IFN gamma. Design and methods: Skin biopsies from lesional and non-lesional skin of 30 patients and 10 healthy controls were obtained for quantitative PCR examination of IL-12 (P40) and IFN-gamma rnRNA as well as in situ PCR of IL-12 (P40) and IFN-gamma mRNA. Results: IL-12 and IFN-gamma levels were higher in lesional skin than in non-lesional and control skin. A significant correlation between IL-12 and IFN gamma was found. By in situ PCR hybridization, IL-12 expression was only found in the dermis, while IFN-gamma was invariably expressed in the dermis and/or epidermis. Conclusion: We suggest that IL-12 independently and through IFN gamma induction may have a crucial role in the development of the active psoriatic lesion itself. where it is probably produced locally in the dermis as a step in the evolution of the psoriatic lesion. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved.

引用

页码：119 / 125

页数：7

共 38 条

[1] The majority of epidermal T cells in Psoriasis vulgaris lesions can produce type 1 cytokines, interferon-γ, interleukin-2, and tumor necrosis factor-α, defining TC1 (cytotoxic T lymphocyte) and TH1 effector populations:: a type 1 differentiation bias is also measured in circulating blood T cells in psoriatic patients [J].