Brazilin Inhibits Growth and Induces Apoptosis in Human Glioblastoma Cells

被引：51

作者：

Lee, Dae-Young ^{[1
]}

Lee, Mi-Kyoung ^{[2
]}

Kim, Geum-Soog ^{[1
]}

Noh, Hyung-Jun ^{[1
]}

Lee, Min-Ho ^{[3
,4
]}

机构：

[1] Natl Inst Hort & Herbal Sci, RDA, Herbal Crop Utilizat Res Team, Eumseong 369873, South Korea

[2] Ajou Univ, Sch Med, Dept Neurosurg, Suwon 443721, South Korea

[3] Kyung Hee Univ, Grad Sch Biotechnol, Yongin 446701, South Korea

[4] Kyung Hee Univ, Dept Oriental Med Mat & Proc, Yongin 446701, South Korea

来源：

MOLECULES | 2013年 / 18卷 / 02期

关键词：

apoptosis; brazilin; Caesalpinia sappan; glioma U87; BRAIN;

D O I：

10.3390/molecules18022449

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Brazilin, isolated from the methanol extract of the heart wood of Caesalpinia sappan, sensitizes cancer cells to apoptosis. Glioblastoma multiforme (GBM), which accounts for most cases of central nervous system malignancy, has a very poor prognosis and lacks effective therapeutic interventions. We, therefore, investigated the effects of different concentrations of and different periods of exposure to brazilin on cell proliferation and apoptosis in the glioma U87 cell line. Cell proliferation was investigated by MTT assays and growth curve analysis, apoptosis was assessed by FACS analysis and western blot studies. Brazilin showed dose-dependent inhibition of cell proliferation and induction of apoptosis in glioma cells. It also increased the ratio of cleaved poly-(ADP)-ribose polymerase and decreased the expression of caspase-3 and caspase-7.

引用

页码：2449 / 2457

页数：9

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