Characterization of clonal relatedness among the natural population of Staphylococcus aureus strains by using spa sequence typing and the BURP (based upon repeat patterns) algorithm

被引:78
|
作者
Mellmann, Alexander [1 ]
Weniger, Thomas [2 ]
Berssenbruegge, Christoph [1 ]
Keckevoet, Ursula [1 ]
Friedrich, Alexander W. [1 ]
Harmsen, Dag [2 ]
Grundmann, Hajo [3 ]
机构
[1] Univ Hosp Munster, Inst Hyg, D-48149 Munster, Germany
[2] Univ Hosp Munster, Dept Periodontol, D-48149 Munster, Germany
[3] Natl Inst Publ Hlth & Environm, Ctr Infect Dis Epidemiol, NL-3720 BA Bilthoven, Netherlands
关键词
D O I
10.1128/JCM.00071-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the BURP (based upon repeat patterns) algorithm, which relies on sequencing of the Staphylococcus aureus protein A gene (spa), for its ability to infer clonal relatedness within a population of 110 wild-type strains. BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%).
引用
收藏
页码:2805 / 2808
页数:4
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