Regulation of nitric oxide-induced apoptosis by sensitive to apoptosis gene protein

被引:14
作者
Yang, ES [1 ]
Park, JW [1 ]
机构
[1] Kyungpook Natl Univ, Coll Nat Sci, Sch Life Sci & Biotechnol, Taegu 702701, South Korea
基金
新加坡国家研究基金会;
关键词
SAG; nitric oxide; U937; apoptosis; antioxidant protein;
D O I
10.1080/10715760500511500
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sensitive to apoptosis gene (SAG) protein, a novel zinc RING finger protein that protects mammalian cells from apoptosis by redox reagents, is a metal chelator and a potential reactive oxygen species (ROS) scavenger, but its antioxidant properties have not been completely defined. Nitric oxide (NO), a radical species produced by many types of cells, is known to play a critical role in many regulatory processes, yet it may also participate in collateral reactions at higher concentrations, leading to cellular oxidative stress. In this report, we demonstrate that modulation of SAG expression in U937 cells regulates NO-induced apoptosis. When we examined the protective role of SAG against NO-induced apoptosis with U937 cells transfected with the cDNA for SAG, a clear inverse relationship was observed between the amount of SAG expressed in target cells and their susceptibility to apoptosis. We also observed the significant decrease in the endogenous production of ROS and oxidative DNA damage in SAG-overexpressed cells compared to control cells upon exposure to NO. These results suggest that SAG plays an important protective role in NO-induced apoptosis, presumably, through regulating the cellular redox status.
引用
收藏
页码:279 / 284
页数:6
相关论文
共 23 条
[1]   INACTIVATION OF GLUTATHIONE-PEROXIDASE BY NITRIC-OXIDE - IMPLICATION FOR CYTOTOXICITY [J].
ASAHI, M ;
FUJII, J ;
SUZUKI, K ;
SEO, HG ;
KUZUYA, T ;
HORI, M ;
TADA, M ;
FUJII, S ;
TANIGUCHI, N .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (36) :21035-21039
[2]   Nitric-oxide-induced necrosis and apoptosis in PC12 cells mediated by mitochondria [J].
Bal-Price, A ;
Brown, GC .
JOURNAL OF NEUROCHEMISTRY, 2000, 75 (04) :1455-1464
[3]   SUBCOMPARTMENTS OF THE G1 PHASE OF CELL-CYCLE DETECTED BY FLOW CYTOMETRY [J].
DARZYNKIEWICZ, Z ;
SHARPLESS, T ;
STAIANOCOICO, L ;
MELAMED, MR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (11) :6696-6699
[4]  
Duan HJ, 1999, MOL CELL BIOL, V19, P3145
[5]   Human sensitive to apoptosis gene protein inhibits peroxynitrite-induced DNA damage [J].
Kim, SY ;
Lee, JH ;
Yang, ES ;
Kil, IS ;
Park, JW .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 301 (03) :671-674
[6]   Thiol-linked peroxidase activity of human sensitive to apoptosis gene (SAG) protein [J].
Kim, SY ;
Bae, YS ;
Park, JW .
FREE RADICAL RESEARCH, 2002, 36 (01) :73-78
[7]   Cytosolic NADP+-dependent isocitrate dehydrogenase status modulates oxidative damage to cells [J].
Lee, SM ;
Koh, HJ ;
Park, DC ;
Song, BJ ;
Huh, TL ;
Park, JW .
FREE RADICAL BIOLOGY AND MEDICINE, 2002, 32 (11) :1185-1196
[8]   NO-induced oxidative stress and glutathione metabolism in rodent and human cells [J].
Luperchio, S ;
Tamir, S ;
Tannenbaum, SR .
FREE RADICAL BIOLOGY AND MEDICINE, 1996, 21 (04) :513-519
[9]   Nitric oxide-induced oxidant stress in endothelial cells: amelioration by ascorbic acid [J].
May, JM ;
Qu, ZC .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2004, 429 (01) :106-113
[10]  
MESSMER UK, 1994, FEBS LETT, V355, P23