Development and Application of a Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Porcine Circovirus 2

被引：22

作者：

Ge, Meng ^{[1
]}

Luo, Wei ^{[1
]}

Jiang, Daliang ^{[1
]}

Li, Runcheng ^{[1
]}

Zhao, Wenwei ^{[1
]}

Chen, Guoliang ^{[1
]}

Yang, Xingdong ^{[1
]}

Yu, Xinglong ^{[1
]}

机构：

[1] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2012年 / 19卷 / 09期

基金：

中国国家自然科学基金;

关键词：

MULTISYSTEMIC WASTING SYNDROME; RESPIRATORY SYNDROME VIRUS; PROTEIN-BASED ELISA; CAPSID PROTEIN; ORF2; PROTEIN; TYPE-2; SWINE; PIGS; INFECTION; PCV2;

D O I：

10.1128/CVI.00234-12

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the diagnostic sensitivity, specificity, and accuracy of the assay were, respectively, 90.61, 94.02, and 91.62% compared with IIF and 94.38, 95.28, and 94.67% compared with the commercial ELISA kit. Assay of 12 PCV-free pigs over a 5-week period produced only PCV2-negative titers by all 3 methods. These results and the seroprofiles of 4 pig farms obtained by both the commercial ELISA kit and the double-antigen sandwich ELISA indicate that the sandwich ELISA is a reliable method for detection of antibodies to PCV2. Additionally, the method described here permits the use of undiluted test serum samples simultaneously loaded with horseradish peroxidase (HRP)-conjugated antigen into the test well, and the complete test procedure can be performed in less than 90 min. This double-antigen sandwich ELISA should be a useful tool to aid swine industry professionals in deciding the intervention strategies for the control of PCV2-associated diseases.

引用

页码：1480 / 1486

页数：7

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