Functional characterization of the histidine kinase of the E-coli two-component signal transduction system AtoS-AtoC

被引:20
|
作者
Filippou, Panagiota S. [1 ]
Kasemian, Lucy D. [1 ]
Panagiotidis, Christos A. [2 ]
Kyriakidis, Dimitrios A. [1 ,3 ]
机构
[1] Aristotle Univ Thessaloniki, Dept Chem, Biochem Lab, Thessaloniki 54124, Greece
[2] Aristotle Univ Thessaloniki, Dept Pharmaceut Sci, Thessaloniki 54124, Greece
[3] Natl Hellen Res Fdn, Athens 11635, Greece
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2008年 / 1780卷 / 09期
关键词
AtoS; AtoC; antizyme; atoDAEB; two-component system; histidine kinase; phosphorylation;
D O I
10.1016/j.bbagen.2008.05.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Escherichia coli AtoS-AtoC two-component signal transduction system regulates the expression of the atoDAEB operon genes, whose products are required for short-chain fatty acid catabolism. In this Study purified his-tagged wild-type and Mutant AtoS proteins were used to prove that these proteins are true sensor kinases. The phosphorylated residue was identified as the histidine-398, which was located in a conserved H-box since AtoS carrying a mutation at this site failed to phosphorylate. This inability to phosphorylate was not due to gross structural alterations of AtoS since the H398L mutant retained its capability to bind ATP. Furthermore, the H398L mutant AtoS was competent to catalyze the transphosphorylation of an AtoS G-box (G565A) mutant protein which otherwise failed to autophosphorylate due to its inability to bind ATP. The formation of homodimers between the various AtoS proteins was also shown by cross-linking experiments both in vitro and in vivo. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:1023 / 1031
页数:9
相关论文
共 50 条
  • [31] Physical Models of transcription factors activated via histidine kinase two-component signal transduction signaling pathways
    O'Mara, Deirdre E.
    Stock, Ann
    Herman, Tim
    Feng, Ashley
    Hanchuk, Stephanie
    Oliveira, Vitor
    Kolb, Catherine
    Wei, Victoria
    Young, Andrew
    Contess, Susan
    FASEB JOURNAL, 2009, 23
  • [32] Transcriptional regulation of the two-component system DcuS-R operon of E-coli
    Oyamada, Tomoya
    Yokoyama, Katsushi
    Makino, Kozo
    GENES & GENETIC SYSTEMS, 2006, 81 (06) : 435 - 435
  • [33] Activation of the AtoSC two-component system in the absence of the AtoC N-terminal receiver domain in E. coli
    Evaggelos C. Theodorou
    Marina C. Theodorou
    Margarita N. Samali
    Dimitrios A. Kyriakidis
    Amino Acids, 2011, 40 : 421 - 430
  • [34] The arc two-component signal transduction system inhibits in vitro Escherichia coli chromosomal initiation
    Lee, YS
    Han, JS
    Jeon, Y
    Hwang, DS
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (13) : 9917 - 9923
  • [35] Activation of the AtoSC two-component system in the absence of the AtoC N-terminal receiver domain in E. coli
    Theodorou, Evaggelos C.
    Theodorou, Marina C.
    Samali, Margarita N.
    Kyriakidis, Dimitrios A.
    AMINO ACIDS, 2011, 40 (02) : 421 - 430
  • [36] Molecular characterization of the PhoP/PhoQ two-component signal transduction system in Erwinia amylovora
    Nakka, S.
    Zhao, Y.
    PHYTOPATHOLOGY, 2008, 98 (06) : S111 - S112
  • [37] Cytokinin signal transduction, involvement of a two-component system.
    Kakimoto, T
    Kubo, M
    Shindo, M
    PLANT PHYSIOLOGY, 1997, 114 (03) : 40003 - 40003
  • [38] A developmentally regulated two-component signal transduction system in Chlamydia
    Koo, IC
    Stephens, RS
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (19) : 17314 - 17319
  • [39] An essential two-component signal transduction system in Mycobacterium tuberculosis
    Zahrt, TC
    Deretic, V
    JOURNAL OF BACTERIOLOGY, 2000, 182 (13) : 3832 - 3838
  • [40] Mechanism of signal transduction in a two-component system formed by a globin-coupled histidine kinase, AfGcHK (a heme-based oxygen sensor)
    Lengalova, A.
    Mihalcin, P.
    Skalova, T.
    Kolenko, P.
    Dohnalek, J.
    Stranava, M.
    Martinkova, M.
    FEBS OPEN BIO, 2019, 9 : 126 - 126