Synthesis, characterization, cytotoxicity, apoptotic inducing activity, cellular uptake, interaction of DNA binding and antioxidant activity studies of ruthenium(II) complexes

被引：31
作者：
Liu, Yun-Jun [1 ]
Liang, Zhen-Hua [1 ]
Hong, Xian-Lan [2 ]
Li, Zheng-Zheng [1 ]
Yao, Jun-Hua [3 ]
Huang, Hong-Liang [4 ]
机构：
[1] Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Guangdong, Peoples R China
[2] Shaoguan Univ, Dept Chem, Shaoguan 512005, Peoples R China
[3] Sun Yat Sen Univ, Instrumentat Anal & Res Ctr, Guangzhou 510275, Guangdong, Peoples R China
[4] Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou 510006, Guangdong, Peoples R China
来源：
INORGANICA CHIMICA ACTA | 2012年 / 387卷
关键词：
Ruthenium(II) complexes; DNA-binding; Cytotoxicity; Apoptosis; Cellular uptake; Antioxidant activity; DEOXYRIBONUCLEIC-ACID; POLYPYRIDYL COMPLEXES; IN-VITRO; TRIS(PHENANTHROLINE)RUTHENIUM(II); CLEAVAGE; LIGAND;
D O I：
10.1016/j.ica.2012.01.003
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
Two new ligands APIP, HAPIP and their relative ruthenium(II) complexes [Ru(bpy)(2)(APIP)](ClO4)(2) 1 and [Ru(bpy)(2)(HAPIP)](ClO4)(2) 2 have been synthesized and characterized. The DNA binding constants for complexes 1 and 2 have been determined to be 6.08 (+/-0.29) x 10(4) M (1) and 1.78 (+/-0.30) x 10(5) M (1). The results suggest that these complexes intercalate between the base pairs of DNA. The cytotoxicity of complexes has been evaluated by MTT assay. The apoptosis assay was carried out with AO/EB staining methods. The cellular uptake was observed under fluorescence microscopy. The studies on the mechanism of photocleavage demonstrate that superoxide anion radical (O-2(center dot)) and singlet oxygen (O-1(2)) may play an important role. The antioxidant activity against hydroxyl radical ((OH)-O-center dot) was also studied. (C) 2012 Elsevier B. V. All rights reserved.
引用
收藏
页码：117 / 124
页数：8
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