Cre-mediated recombination in mouse Clara cells

被引：26

作者：

Li, HuaiGuang ^{[1
]}

Cho, Sung Nam ^{[1
]}

Evans, Christopher M. ^{[2
]}

Dickey, Burton F. ^{[3
]}

Jeong, Jae-Wook ^{[1
]}

DeMayo, Francesco J. ^{[1
]}

机构：

[1] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA

[2] Univ Texas MD Anderson Canc Ctr, Div Internal Med, Houston, TX 77030 USA

[3] Univ Texas MD Anderson Canc Ctr, Dept Pulm Med, Houston, TX 77030 USA

来源：

GENESIS | 2008年 / 46卷 / 06期

关键词：

CCSP; lung; Cre recombinase; Clara cell; knock-in;

D O I：

10.1002/dvg.20396

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Clara cells are nonciliated secretory cells lining the respiratory epithelium and are easily identified by the expression of Clara cell secretory protein (CCSP). To investigate molecular mechanism(s) regulating Clara cell function in the lungs, Cre recombinase was inserted into exon 1 of the CCSP, generating two novel mouse models, CCSPCre-Neo and CCSPCre. These two models differ only by the inclusion of the neomycin resistance gene. These mice were bred to the R26R reporter mouse to investigate the tissue and cell specificity of Cre-mediated recombination. The efficiency of Cre recombination in the CCSPCre mouse model was higher than in the CCSPCre-Neo mouse model. Recombination was detected at D 4.5 in CCSPCre-Neo/R26R mice and at D 0.5 in CCSPCre/R26R mice. The CCSPCre-Neo and CCSPCre mouse models provide valuable tools for the ablation of genes in the postnatal mouse Clara cells.

引用

页码：300 / 307

页数：8

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