Aflatoxin B1 induces Src phosphorylation and stimulates lung cancer cell migration

被引:23
作者
Cui, Anguo [1 ]
Hua, Hui [2 ]
Shao, Ting [1 ]
Song, Peiying [1 ]
Kong, Qingbin [1 ]
Luo, Ting [3 ]
Jiang, Yangfu [1 ]
机构
[1] Sichuan Univ, W China Hosp, Sect Oncogene, State Key Lab Biotherapy, Chengdu 610041, Peoples R China
[2] Sichuan Univ, W China Hosp, Lab Stem Cell Biol, Chengdu 610041, Peoples R China
[3] Sichuan Univ, W China Hosp, Ctr Canc, Chengdu 610041, Peoples R China
基金
中国国家自然科学基金;
关键词
Aflatoxin B1; IRS; Src; Lung cancer; Cell migration; INSULIN; B-1; ACTIVATION; EXPRESSION; BIOTRANSFORMATION; TRANSFORMATION; PROLIFERATION; METABOLISM; DEPLETION; KINASES;
D O I
10.1007/s13277-015-3341-2
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
AflatoxinB1 (AFB1) is well known as a potent carcinogen. Epidemiological studies have shown an association between AFB1 exposure and lung cancer in humans. AFB1 can induce the mutations of genes such as tumor suppressor p53 through its metabolite AFB1-8,9-exo-epoxide, which acts as a mutagen to react with DNA. In addition, recent study demonstrates AFB1 positively regulates type I insulin-like growth factor receptor (IGF-IR) signaling in hepatoma cells. The current study aims to determine the effects of AFB1 on Src kinase and insulin receptor substrate (IRS) in lung cancer cells and the effects of AFB1 on lung cancer cell migration. To this end, the effects of AFB1 on IRS expression, Src, Akt, and ERK phosphorylation were measured by Western blot analysis. The migration of lung cancer cells was detected by wound-healing assay. AFB1 downregulates IRS1 but paradoxically upregulates IRS2 through positive regulation of the stability of IRS2 and the proteasomal degradation of IRS1 in lung cancer cell lines A549 and SPCA-1. In addition, AFB1 induces Src, Akt, and ERK1/2 phosphorylation. Treatment of lung cancer cells with Src inhibitor saracatinib abrogates AFB1-induced IRS2 accumulation. Moreover, AFB1 stimulates lung cancer cell migration, which can be inhibited by saracatinib. We conclude that AFB1 may upregulate IRS2 and stimulate lung cancer cell migration through Src.
引用
收藏
页码:6507 / 6513
页数:7
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