Rapid liquid chromatographic determination of oxytetracycline in milk

被引:41
作者
Furusawa, N [1 ]
机构
[1] Osaka City Univ, Fac Human Life Sci, Sumiyoshi Ku, Osaka 5588585, Japan
关键词
oxytetracycline; antibiotics;
D O I
10.1016/S0021-9673(99)00150-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple method for the determination of residual oxytetracycline (OTC) in milk by high-performance liquid chromatography (HPLC) was developed. The sample preparation could be made without complex extraction and clean-up procedures. A LiChrospher 100 RP-8 end-capped column and a mobile phase of acetonitrile-acetic acid-water (28:4.68, v/v/v) with a photo-diode array detector was used. The average recoveries from spiked OTC (0.1, 0.5 and 1.0 mu/ml) were in excess of 89.8% with coefficients of variation between 0.6 and 4.1%. The limit of detection was 0.05 mu g/ml. The total time required for the analysis of one sample was below 10 min. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:247 / 251
页数:5
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