Putative drug and vaccine target protein identification using comparative genomic analysis of KEGG annotated metabolic pathways of Mycoplasma hyopneumoniae

被引：35

作者：

Damte, Dereje ^{[1
]}

Suh, Joo-Won ^{[2
,3
]}

Lee, Seung-Jin ^{[1
]}

Yohannes, Sileshi Belew ^{[1
]}

Hossain, Md. Akil ^{[1
]}

Park, Seung-Chun ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Lab Vet Pharmacokinet & Pharmacodynam, Coll Vet Med, Taegu 702701, South Korea

[2] Myongji Univ, Ctr Nutraceut & Pharmaceut Mat, Yongin 449728, Gyeonggi, South Korea

[3] Myongji Univ, Div Biosci & Bioinformat, Yongin 449728, Gyeonggi, South Korea

来源：

GENOMICS | 2013年 / 102卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Mycoplasma hyopneumoniae; KEGG metabolic pathways; Therapeutic targets; Vaccine candidate; ESSENTIAL GENES; PREDICTION; DEHYDROGENASE; SEQUENCE; PRIORITIZATION; STREPTOCOCCUS; DATABASE; SWINE; DEG;

D O I：

10.1016/j.ygeno.2013.04.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present study, a computational comparative and subtractive genomic/proteomic analysis aimed at the identification of putative therapeutic target and vaccine candidate proteins from Kyoto Encyclopedia of Genes and Genomes (KEGG) annotated metabolic pathways of Mycoplasma hyopneumoniae was performed for drug design and vaccine production pipelines against M.hyopneumoniae. The employed comparative genomic and metabolic pathway analysis with a predefined computational systemic workflow extracted a total of 41 annotated metabolic pathways from KEGG among which five were unique to M. hyopneumoniae. A total of 234 proteins were identified to be involved in these metabolic pathways. Although 125 non homologous and predicted essential proteins were found from the total that could serve as potential drug targets and vaccine candidates, additional prioritizing parameters characterize 21 proteins as vaccine candidate while druggability of each of the identified proteins evaluated by the DrugBank database prioritized 42 proteins suitable for drug targets. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：47 / 56

页数：10

共 46 条

[1] Genomic-scale prioritization of drug targets: the TDR Targets database [J].

Agueero, Fernan ;

Al-Lazikani, Bissan ;

Aslett, Martin ;

Berriman, Matthew ;

Buckner, Frederick S. ;

Campbell, Robert K. ;

Carmona, Santiago ;

Carruthers, Ian M. ;

Chan, A. W. Edith ;

Chen, Feng ;

Crowther, Gregory J. ;

Doyle, Maria A. ;

Hertz-Fowler, Christiane ;

Hopkins, Andrew L. ;

McAllister, Gregg ;

Nwaka, Solomon ;

Overington, John P. ;

Pain, Arnab ;

Paolini, Gala V. ;

Pieper, Ursula ;

Ralph, Stuart A. ;

Riechers, Aaron ;

Roos, David S. ;

Sali, Andrej ;

Shanmugam, Dhanasekaran ;

Suzuki, Takashi ;

Van Voorhis, Wesley C. ;

Verlinde, Christophe L. M. J. .

NATURE REVIEWS DRUG DISCOVERY, 2008, 7 (11) :900-907

[2]

Amineni U., 2010, J CHEM BIOL, V14, P165

[3] Announcing the worldwide Protein Data Bank [J].

Berman, H ;

Henrick, K ;

Nakamura, H .

NATURE STRUCTURAL BIOLOGY, 2003, 10 (12) :980-980

[4] The worldwide Protein Data Bank (wwPDB): ensuring a single, uniform archive of PDB data [J].

Berman, Helen ;

Henrick, Kim ;

Nakamura, Haruki ;

Markley, John L. .

NUCLEIC ACIDS RESEARCH, 2007, 35 :D301-D303

[5] The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003 [J].

Boeckmann, B ;

Bairoch, A ;

Apweiler, R ;

Blatter, MC ;

Estreicher, A ;

Gasteiger, E ;

Martin, MJ ;

Michoud, K ;

O'Donovan, C ;

Phan, I ;

Pilbout, S ;

Schneider, M .

NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :365-370

[6] Comparative Genomics Analysis of Mycobacterium ulcerans for the Identification of Putative Essential Genes and Therapeutic Candidates [J].

Butt, Azeem Mehmood ;

Nasrullah, Izza ;

Tahir, Shifa ;

Tong, Yigang .

PLOS ONE, 2012, 7 (08)

[7] Mycoplasma genitalium: A comparative genomics study of metabolic pathways for the identification of drug and vaccine targets [J].

Butt, Azeem Mehmood ;

Tahir, Shifa ;

Nasrullah, Izza ;

Idrees, Muhammad ;

Lu, Jun ;

Tong, Yigang .

INFECTION GENETICS AND EVOLUTION, 2012, 12 (01) :53-62

[8]

Chong Chan-Eng, 2006, In Silico Biology, V6, P341

[9] Surfome Analysis as a Fast Track to Vaccine Discovery [J].

Doro, Francesco ;

Liberatori, Sabrina ;

Rodriguez-Ortega, Manuel J. ;

Rinaudo, Cira D. ;

Rosini, Roberto ;

Mora, Marirosa ;

Scarselli, Maria ;

Altindis, Emrah ;

D'Aurizio, Romina ;

Stella, Maria ;

Margarit, Immaculada ;

Maione, Domenico ;

Telford, John L. ;

Norais, Nathalie ;

Grandi, Guido .

MOLECULAR & CELLULAR PROTEOMICS, 2009, 8 (07) :1728-1737

[10] Drug target prediction and prioritization: using orthology to predict essentiality in parasite genomes [J].

Doyle, Maria A. ;

Gasser, Robin B. ;

Woodcroft, Ben J. ;

Hall, Ross S. ;

Ralph, Stuart A. .

BMC GENOMICS, 2010, 11

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