Cell Survival within Pulp and Periodontal Constructs

被引：30
作者：
Gebhardt, Matthew [2 ]
Murray, Peter E. [1 ]
Namerow, Kenneth N. [1 ]
Kuttler, Sergio [1 ]
Garcia-Godoy, Franklin [1 ]
机构：
[1] Nova SE Univ, Coll Dent Med, Dept Endodont, Ft Lauderdale, FL 33328 USA
[2] Private Practice, Medford, OR USA
来源：
JOURNAL OF ENDODONTICS | 2009年 / 35卷 / 01期
关键词：
Endodontics; regenerative; scaffolds; stem cells; tissue engineering; STEM-CELLS; APICAL PERIODONTITIS; POLYMER SCAFFOLDS; TISSUE; TEETH; TOOTH; REVASCULARIZATION; ENDODONTICS; PHOSPHATE; LIGAMENT;
D O I：
10.1016/j.joen.2008.09.020
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The purpose of this study was to measure cell survival and degradation within tissue-engineered dental constructs. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were seeded on three types of tissue engineering scaffolds: a synthetic open-cell D,D-L,L-polylactic acid (polymer) scaffold, a bovine collagen scaffold (collagen), and a calcium phosphate bioceramic (calcium phosphate) scaffold. The dental pulp and periodontal constructs (n = 144) were maintained in cell culture for between 3 and 14 days. The cell survival and degradation within the constructs were measured using histologic criteria, The DPSC and PLSC survival was optimal in the polymer and collagen constructs but not the calcium phosphate constructs, especially over longer time periods. These in vitro results suggest that both the polymer and collagen scaffolds and the DPSCs and PLSCs can be combined to create pulp and periodontal constructs for use in future regenerative dental treatments. (J Endod 2009;35:63- 66)
引用
收藏
页码：63 / 66
页数：4
相关论文
共 30 条
[1]   Revascularization of immature permanent teeth with apical periodontitis: New treatment protocol? [J].
Banchs, F ;
Trope, M .
JOURNAL OF ENDODONTICS, 2004, 30 (04) :196-200
[2]   Fabrication and in vitro characterization of porous biodegradable composites based on phosphate glasses and oligolactide-containing polymer networks [J].
Brauer, Delia S. ;
Ruessel, Christian ;
Vogt, Sebastian ;
Weisser, Juergen ;
Schnabelrauch, Matthias .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 80A (02) :410-420
[3]   Immature teeth with periradicular periodontitis or abscess undergoing apexogenesis: A paradigm shift [J].
Chueh, Ling-Huey ;
Huang, George T. J. .
JOURNAL OF ENDODONTICS, 2006, 32 (12) :1205-1213
[4]   Biomimetic tissue-engineered anterior cruciate ligament replacement [J].
Cooper, James A., Jr. ;
Sahota, Janmeet S. ;
Gorum, W. Jay, II ;
Carter, Janell ;
Doty, Stephen B. ;
Laurencin, Cato T. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (09) :3049-3054
[5]   Bioengineered teeth from cultured rat tooth bud cells [J].
Duailibi, MT ;
Duailibi, SE ;
Young, CS ;
Bartlett, JD ;
Vacanti, JP ;
Yelick, PC .
JOURNAL OF DENTAL RESEARCH, 2004, 83 (07) :523-528
[6]   Bioactive materials in endodontics [J].
Enkel, Benedicte ;
Dupas, Cecile ;
Armengol, Valerie ;
Adou, Jonas Akpe ;
Bosco, Julia ;
Daculsi, Guy ;
Jean, Alain ;
Laboux, Olivier ;
LeGeros, Racquel Z. ;
Weiss, Pierre .
EXPERT REVIEW OF MEDICAL DEVICES, 2008, 5 (04) :475-494
[7]   Stem cells and adipose tissue engineering [J].
Gomillion, Cheryl T. ;
Burg, Karen J. L. .
BIOMATERIALS, 2006, 27 (36) :6052-6063
[8]   An ultrastructural investigation of tissue-engineered pulp constructs implanted within endodontically treated teeth [J].
Gotileb, Eric L. ;
Murray, Peter E. ;
Namerow, Kenneth N. ;
Kuttler, Sergio ;
Garcia-Godoy, Franklin .
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, 2008, 139 (04) :457-465
[9]   Stem cell properties of human dental pulp stem cells [J].
Gronthos, S ;
Brahim, J ;
Li, W ;
Fisher, LW ;
Cherman, N ;
Boyde, A ;
DenBesten, P ;
Robey, PG ;
Shi, S .
JOURNAL OF DENTAL RESEARCH, 2002, 81 (08) :531-535
[10]   TISSUE FORMATION IN ROOT-CANAL AFTER TOTAL PULPECTOMY AND PARTIAL ROOT FILLING [J].
HORSTED, P ;
NYGAARDOSTBY, B .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 1978, 46 (02) :275-282
123