Referenced luminescent sensing and imaging with digital color cameras: A comparative study

被引:58
作者
Meier, Robert J. [1 ]
Fischer, Lorenz H. [1 ]
Wolfbeis, Otto S. [1 ]
Schaeferling, Michael [1 ]
机构
[1] Univ Regensburg, Inst Analyt Chem Chemo & Biosensors, D-93040 Regensburg, Germany
关键词
Chemical sensor; Optical sensor; Luminescence; CCD; CMOS; RGB camera; Pressure sensitive paint; TEMPERATURE-SENSITIVE PAINT; OPTICAL CHEMICAL SENSORS; PH IN-VIVO; READ-OUT; PRESSURE; OXYGEN; NANOPARTICLES; DEVICES; PROBE;
D O I
10.1016/j.snb.2012.11.041
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We have performed a comparative study on different imaging techniques for optical chemical sensors with the aim to assess the utility of red-green-blue (RGB) color cameras for quantitative analysis. A luminescent film for sensing barometric pressure (via quenching by oxygen) was used as a model system and calibrated by four fluorescence imaging methods including intensity imaging, referenced intensity imaging, lifetime imaging, and RGB based imaging using a customary digital color camera. The results are compared with respect to standard deviations, lateral signal homogeneity, and resolution. The imaging methods were applied to the sensor film under identical experimental conditions in order to ensure comparable results. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:500 / 506
页数:7
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