Probing neuronal activities with genetically encoded optical indicators: from a historical to a forward-looking perspective

被引：13
作者：
Mutoh, Hiroki [1 ]
Knoepfel, Thomas [1 ]
机构：
[1] RIKEN Brain Sci Inst, Wako, Saitama 3510198, Japan
来源：
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 2013年 / 465卷 / 03期
基金：
美国国家卫生研究院;
关键词：
Optical imaging; Genetically encoded indicators; Calcium; Membrane potential; INTRACELLULAR CALCIUM-CONCENTRATION; ACTIVITY-INDUCED ELEVATIONS; FLUORESCENT VOLTAGE SENSOR; SYNAPTIC-TRANSMISSION; CA2+ INDICATORS; NEURAL ACTIVITY; CONFORMATIONAL-CHANGES; LIVING CELLS; FRET; EXPRESSION;
D O I：
10.1007/s00424-012-1202-z
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
Optical imaging has a long history in physiology and in neurophysiology in particular. Over the past 15 years or so, new methodologies have emerged that combine genetic engineering with light-based imaging methods. This merger has resulted in a tool box of genetically encoded optical indicators that enable nondestructive and long-lasting monitoring of neuronal activities at the cellular, circuit, and system level. This review describes the historical roots and fundamental concepts underlying these new approaches, evaluates current progress in this field, and concludes with a forward-looking perspective on current work and potential future developments in this field.
引用
收藏
页码：361 / 371
页数：11
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