Metabolomics Comparison of Hanwoo (Bos taurus coreanae) Biofluids Using Proton Nuclear Magnetic Resonance Spectroscopy

被引：5

作者：

Eom, Jun Sik ^{[1
]}

Lee, Shin Ja ^{[2
,3
]}

Kim, Hyun Sang ^{[1
]}

Choi, You Young ^{[1
]}

Kim, Sang Ho ^{[4
]}

Lee, Yoo Gyung ^{[4
]}

Lee, Sung Sill ^{[1
,2
,3
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci BK21Plus, Gyeongsangnam Do 52828, Jinju Si, South Korea

[2] Gyeongsang Natl Univ, Inst Agr & Life Sci, Gyeongsangnam Do, Jinju Si, South Korea

[3] Gyeongsang Natl Univ, Univ Ctr Labs, Gyeongsangnam Do 52828, Jinju Si, South Korea

[4] Natl Inst Anim Sci, RDA, Anim Nutr & Physiol Team, Jeonrabuk Do 55365, Jeonju Si, South Korea

来源：

METABOLITES | 2020年 / 10卷 / 08期

关键词：

H-1-NMR spectroscopy; biofluids; Bos taurus coreanae; Hanwoo; metabolomic profiles; DAIRY-COWS; ENERGY-BALANCE; METHANE PRODUCTION; FATTY-ACIDS; MILK; FERMENTATION; CHOLINE; PROFILE; DIET; METHANOGENESIS;

D O I：

10.3390/metabo10080333

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to identify the metabolomic profiles of rumen fluid, serum, and urine from Hanwoo (Bos taurus coreanae), using proton nuclear magnetic resonance (H-1-NMR) spectroscopy. In all, 189, 110, and 188 metabolites were identified in rumen fluid, serum, and urine, and 107, 49, and 99 were quantified, respectively. Organic acids, carbohydrates, and aliphatic acyclic compound metabolites were present at the highest concentrations in rumen fluid, serum, and urine, respectively. In addition, acetate, glucose, and urea were the most highly concentrated individual metabolites in rumen fluid, serum, and urine, respectively. In all, 77 metabolites were commonly identified, and 19 were quantified across three biofluids. Metabolic pathway analysis showed that the common quantified metabolites could provide relevant information about three main metabolic pathways, phenylalanine, tyrosine, and tryptophan biosynthesis; caffeine metabolism; and histidine metabolism. These results can be useful as reference values for future metabolomic research on Hanwoo biofluids in Korea.

引用

页码：1 / 16

页数：16

共 71 条

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