Meiotic Sex Chromosome Inactivation Is Disrupted in Sterile Hybrid Male House Mice

被引:63
作者
Campbell, Polly [1 ]
Good, Jeffrey M. [2 ]
Nachman, Michael W. [1 ]
机构
[1] Univ Arizona, Dept Ecol & Evolutionary Biol, Tucson, AZ 85721 USA
[2] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
X-CHROMOSOME; Y-CHROMOSOME; CHROMATIN; ASYNAPSIS; TRANSCRIPTION; SPECIATION; REGIONS; ENZYME; GENES; CELLS;
D O I
10.1534/genetics.112.148635
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
In male mammals, the X and Y chromosomes are transcriptionally silenced in primary spermatocytes by meiotic sex chromosome inactivation (MSCI) and remain repressed for the duration of spermatogenesis. Here, we test the longstanding hypothesis that disrupted MSCI might contribute to the preferential sterility of heterogametic hybrid males. We studied a cross between wild-derived inbred strains of Mus musculus musculus and M. m. domesticus in which sterility is asymmetric: F-1 males with a M. m. musculus mother are sterile or nearly so while F-1 males with a M. m. domesticus mother are normal. In previous work, we discovered widespread overexpression of X-linked genes in the testes of sterile but not fertile F-1 males. Here, we ask whether this overexpression is specifically a result of disrupted MSCI. To do this, we isolated cells from different stages of spermatogenesis and measured the expression of several genes using quantitative PCR. We found that X overexpression in sterile F-1 primary spermatocytes is coincident with the onset of MSCI and persists in postmeiotic spermatids. Using a series of recombinant X genotypes, we then asked whether X overexpression in hybrids is controlled by cis-acting loci across the X chromosome. We found that it is not. Instead, one large interval in the proximal portion of the M. m. musculus X chromosome is associated with both overexpression and the severity of sterility phenotypes in hybrids. These results demonstrate a strong association between X-linked hybrid male sterility and disruption of MSCI and suggest that transacting loci on the X are important for the transcriptional regulation of the X chromosome during spermatogenesis.
引用
收藏
页码:819 / +
页数:16
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