Simultaneous Visualization of Endogenous Homocysteine, Cysteine, Glutathione, and their Transformation through Different Fluorescence Channels

被引：187
作者：
Yin, Guoxing [1 ]
Niu, Tingting [2 ]
Yu, Ting [1 ]
Gan, Yabing [1 ]
Sun, Xinyu [1 ]
Yin, Peng [1 ]
Chen, Haimin [2 ]
Zhang, Youyu [1 ]
Li, Haitao [1 ]
Yao, Shouzhuo [1 ]
机构：
[1] Hunan Normal Univ, Key Lab Assembly & Applicat Organ Funct Mol Hunan, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China
[2] Ningbo Univ, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo 315211, Zhejiang, Peoples R China
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2019年 / 58卷 / 14期
基金：
中国国家自然科学基金;
关键词：
endogenous biothiols; fluorescent probe; simultaneous sensing; transformation; HYDROGEN-SULFIDE; B-VITAMINS; PROBES; CYS; GSH; DISCRIMINATION; CYSTEINE/HOMOCYSTEINE; AMINOTHIOLS; DESIGN; PLASMA;
D O I：
10.1002/anie.201813935
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Studying numerous biologically important species simultaneously is crucial to understanding cellular functions and the root causes of related diseases. Direct visualization of endogenous biothiols in biological systems is of great value to understanding their biological roles. Herein, a novel multi-signal fluorescent probe was rationally designed and exploited for the simultaneous sensing of homocysteine (Hcy), cysteine (Cys), and glutathione (GSH) using different emission channels. This probe was successfully applied to the simultaneous discrimination between and visualization of endogenous Hcy, Cys, GSH, and their transformation in living cells.
引用
收藏
页码：4557 / 4561
页数：5
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